Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN400784 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide corresponding to residues surrounding Ser473 of Human AKT1 protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 17F6-B11
- Vial size
- 0.1 mg
- Concentration
- 1.02 mg/ml (by UV absorbance at 280 nm)
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Submitted references Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture.
PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma.
Alessi DR
Biochemical Society transactions 2001 May;29(Pt 2):1-14
Biochemical Society transactions 2001 May;29(Pt 2):1-14
PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
Lawlor MA, Alessi DR
Journal of cell science 2001 Aug;114(Pt 16):2903-10
Journal of cell science 2001 Aug;114(Pt 16):2903-10
Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA
Proceedings of the National Academy of Sciences of the United States of America 1991 May 15;88(10):4171-5
Proceedings of the National Academy of Sciences of the United States of America 1991 May 15;88(10):4171-5
Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma.
Staal SP
Proceedings of the National Academy of Sciences of the United States of America 1987 Jul;84(14):5034-7
Proceedings of the National Academy of Sciences of the United States of America 1987 Jul;84(14):5034-7
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- Image(s): Western Blotting
Supportive validation
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- Image(s): Immunofluorescence
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- Image(s): Immunofluorescence
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunohistochemistry