ABIN362435

antibody from antibodies-online

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362435

Provider

antibodies-online

Product name

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pThr450 (T-I-T (p) -P-P) derived from Human AKT1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pThr450

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Vascular endothelial cell growth factor activates CRE-binding protein by signaling through the KDR receptor tyrosine kinase.

Mayo LD, Kessler KM, Pincheira R, Warren RS, Donner DB
The Journal of biological chemistry 2001 Jul 6;276(27):25184-9

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Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation.

Xing J, Kornhauser JM, Xia Z, Thiele EA, Greenberg ME
Molecular and cellular biology 1998 Apr;18(4):1946-55

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FGF and stress regulate CREB and ATF-1 via a pathway involving p38 MAP kinase and MAPKAP kinase-2.

Tan Y, Rouse J, Zhang A, Cariati S, Cohen P, Comb MJ
The EMBO journal 1996 Sep 2;15(17):4629-42

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Mutation of phosphoserine 389 affects p53 function in vivo.

Hao M, Lowy AM, Kapoor M, Deffie A, Liu G, Lozano G
The Journal of biological chemistry 1996 Nov 15;271(46):29380-5