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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- 10-1010 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-AKT1 Antibody
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant AKT1 p
rotein (amino acids 220-480) was us
ed as the immunogen for this antibo
dy- Isotype
- IgG
- Antibody clone number
- ABM12F7
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of AKT1. Anti- AKT1 antibody (Clone: ABM12F7) was tested at 2 µg/ml on SKBR3, PC3 and BT474.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flowcytometric analysis of AKT1 in HePG2 cell lines using 0.5 µg/10^6 cells of AKT-1 antibody (ABM12F7). Green represent isotype control and red represent Anti-AKT1 antibody (10-1010 Abeomics). Goat Anti-Mouse PE was used as the secondary antibody
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flowcytometric analysis of AKT1 in HeLa cell lines using 0.5 µg/10^6 cells of AKT-1 antibody (ABM12F7). Green represent isotype control and red represent Anti-AKT1 antibody (10-1010 Abeomics). Goat Anti-Mouse PE was used as the secondary antibody
- Protocol
- Protocol
