LS-C775029

antibody from LSBio

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C775029

Provider

LSBio

Product name

AKT1 Antibody (Atto 565) LS-C775029

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human, Mouse

Host

Rabbit

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Mouse Brain showing detection of ~55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa. Other Band(s): Band is detected at ~65 kDa, due to post translational modifications.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-AKT1 Polyclonal Antibody. Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.