H00000207-M03

antibody from Invitrogen Antibodies

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000207-M03

Provider

Invitrogen Antibodies

Product name

AKT1 Monoclonal Antibody (2E11)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2E11

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in NIH/3T3(Cat # L018V1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody (M03), clone 2E11.Lane 1: AKT1 transfected lysate(55.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in PC-12(Cat # L012V1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AKT1 monoclonal antibody (M03), clone 2E11 Western Blot analysis of AKT1 expression in HeLa (Cat # L013V1).

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKT1 is approximately 3 ng/mL as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. Antibody concentration is 20 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration is 3 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKT1 MaxPab rabbit polyclonal antibody.