603401

antibody from BioLegend

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

603401

Provider

BioLegend

Product name

Purified anti-Akt

Antibody type

Polyclonal

Antigen

Recombinant (partial) , C-terminal

Description

Akt (also known as protein kinase B alpha) is a 60 kD serine/threonine specific kinase containing a pleckstrin domain. This kinase is ubiquitously expressed and translocates to the membrane upon activation. Akt phosphorylates substrates in both the cytoplasm and the nucleus. Akt exists as a catalytically inactive multimeric complex until phosphatidyl-3,4,5-triphosphate (PIP3) is generated as a result of cell signaling. Elevated PIP3 results in the partial activation of Akt after phosphorylation by phosphoinositide-dependent protein kinase 1 complex; Akt becomes fully activated only after autophosphorylation. Akt is a potent inhibitor of apoptosis and has been implicated as a factor in tumorigenesis and progression. Survival factors that suppress apoptosis activate Akt which phosphorylates and inactivates components of the apoptotic machinery. Akt can also be activated by TNF, IL-1, PDGF, and insulin like growth factor 1. Akt interacts through its pleckstrin homology domain with the second messengers phosphatidylinositol-(3,4,5)-trisphosphate (PtdIns(3,4,5)P[3]) and PtdIns(3,4). Akt has also been shown to interact with PED/PEA, TCL-1, Nur77, inosine-5'-monophosphate dehydrogenase, and PKC-ζ. The Poly6034 antibody has been shown to be useful for Western blotting of human Akt.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

50 µl

Storage

Upon receipt, store frozen at -20° C.