60203-1-Ig

antibody from Proteintech Group

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

60203-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#60203-1-Ig, RRID:AB_10919160

Product name

AKT1/3 antibody

Antibody type

Monoclonal

Description

KD/KO validated AKT1/3 antibody (Cat. #60203-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

3G5E11

Vial size

20ul, 150ul

References

Submitted references

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Gastroenterology 2023 Sep;165(3):746-761.e16

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The metabolite butyrate produced by gut microbiota inhibits cachexia-associated skeletal muscle atrophy by regulating intestinal barrier function and macrophage polarization.

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International immunopharmacology 2023 Nov;124(Pt B):111001

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PDK1 inhibition reduces autophagy and cell senescence through the PI3K/AKT signalling pathway in a cigarette smoke mouse emphysema model.

Zhang P, Jiang Y, Ye X, Zhang C, Tang Y
Experimental and therapeutic medicine 2023 May;25(5):223

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DSC2 suppresses the growth of gastric cancer through the inhibition of nuclear translocation of γ-catenin and PTEN/PI3K/AKT signaling pathway.

Sun C, Wen K, Zhang B, Dong Y, Chen C, Neo SY, Leng B, Gao TT, Wu J
Aging 2023 Jul 8;15(13):6380-6399

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Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization.

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World journal of surgical oncology 2023 Aug 22;21(1):255

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Babao Dan Reverses Multiple-Drug Resistance in Gastric Cancer Cells via Triggering Apoptosis and Autophagy and Inhibiting PI3K/AKT/mTOR Signaling.

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PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.

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BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.

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Elaiophylin reduces body weight and lowers glucose levels in obese mice by activating AMPK.

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The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway.

Wang B, Zhang M, Guo J, Liu Z, Zhou R, Guo F, Li K, Mu Y
International journal of molecular sciences 2021 Nov 11;22(22)

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Activation of Wnt/β-catenin signaling restores insulin sensitivity in insulin resistant neurons through transcriptional regulation of IRS-1.

Tian S, Tan S, Jia W, Zhao J, Sun X
Journal of neurochemistry 2021 May;157(3):467-478

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Exercise improves lipid droplet metabolism disorder through activation of AMPK-mediated lipophagy in NAFLD.

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miR-320a/SP1 negative reciprocal interaction contributes to cell growth and invasion in colorectal cancer.

Zhang W, Yang H, Wang Z, Wu Y, Wang J, Duan G, Guo Q, Zhang Y
Cancer cell international 2021 Mar 17;21(1):175

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MicroRNA-216a targets WT1 expression and regulates KRT7 transcription to mediate the progression of pancreatic cancer-A transcriptome analysis.

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IUBMB life 2021 Jun;73(6):866-882

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Parthenolide inhibits the tumor characteristics of renal cell carcinoma.

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International journal of oncology 2021 Jan;58(1):100-110

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Treatment of keloids through Runx2 siRNA‑induced inhibition of the PI3K/AKT signaling pathway.

Lv W, Wu M, Ren Y, Luo X, Hu W, Zhang Q, Wu Y
Molecular medicine reports 2021 Jan;23(1)

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RabGEF1 functions as an oncogene in U251 glioblastoma cells and is involved in regulating AKT and Erk pathways.

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Experimental and molecular pathology 2021 Feb;118:104571

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Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 Dec;93:153772

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The orphan nuclear receptor Nurr1 agonist amodiaquine mediates neuroprotective effects in 6-OHDA Parkinson's disease animal model by enhancing the phosphorylation of P38 mitogen-activated kinase but not PI3K/AKT signaling pathway.

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Melatonin prevents diabetes-associated cognitive dysfunction from microglia-mediated neuroinflammation by activating autophagy via TLR4/Akt/mTOR pathway.

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miR-31-5p Regulates 14-3-3 ɛ to Inhibit Prostate Cancer 22RV1 Cell Survival and Proliferation via PI3K/AKT/Bcl-2 Signaling Pathway.

Zhao J, Xu H, Duan Z, Chen X, Ao Z, Chen Y, Ruan Y, Ni M
Cancer management and research 2020;12:6679-6694

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Proanthocyanidin B2 inhibits proliferation and induces apoptosis of osteosarcoma cells by suppressing the PI3K/AKT pathway.

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Journal of cellular and molecular medicine 2020 Oct;24(20):11960-11971

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Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis.

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siPRDX2-elevated DNM3 inhibits the proliferation and metastasis of colon cancer cells via AKT signaling pathway.

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Autophagy Protects the Blood-Brain Barrier Through Regulating the Dynamic of Claudin-5 in Short-Term Starvation.

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Frontiers in physiology 2019;10:2

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NKAP functions as an oncogene in Ewing sarcoma cells partly through the AKT signaling pathway.

Li F, Wu JT, Wang PF, Qu LZ
Experimental and therapeutic medicine 2019 Oct;18(4):3037-3045

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Taurine protects against myelin damage of sciatic nerve in diabetic peripheral neuropathy rats by controlling apoptosis of schwann cells via NGF/Akt/GSK3β pathway.

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International journal of molecular sciences 2019 May 10;20(9)

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Astragaloside IV Regulates the PI3K/Akt/HO-1 Signaling Pathway and Inhibits H9c2 Cardiomyocyte Injury Induced by Hypoxia-Reoxygenation.

Yang P, Zhou Y, Xia Q, Yao L, Chang X
Biological & pharmaceutical bulletin 2019 May 1;42(5):721-727

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Leptin Promotes Monosodium Urate Crystal-Induced Inflammation in Human and Murine Models of Gout.

Yu Y, Yang J, Fu S, Xue Y, Liang M, Xuan D, Zhu X, Wan W, Lv L, Zou H
Journal of immunology (Baltimore, Md. : 1950) 2019 May 1;202(9):2728-2736

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Resveratrol synergizes with low doses of L-DOPA to improve MPTP-induced Parkinson disease in mice.

Liu Q, Zhu D, Jiang P, Tang X, Lang Q, Yu Q, Zhang S, Che Y, Feng X
Behavioural brain research 2019 Jul 23;367:10-18

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Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2.

Hu HY, Yu CH, Zhang HH, Zhang SZ, Yu WY, Yang Y, Chen Q
International journal of biological macromolecules 2019 Jul 1;132:470-477

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Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy.

Wang Q, Sun Y, Li T, Liu L, Zhao Y, Li L, Zhang L, Meng Y
Molecular medicine reports 2019 Jan;19(1):499-507

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Fabrication of high-strength mecobalamin loaded aligned silk fibroin scaffolds for guiding neuronal orientation.

Zhang L, Xu L, Li G, Yang Y
Colloids and surfaces. B, Biointerfaces 2019 Jan 1;173:689-697

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Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice.

Ren Z, Chen L, Wang Y, Wei X, Zeng S, Zheng Y, Gao C, Liu H
Journal of immunology (Baltimore, Md. : 1950) 2019 Feb 1;202(3):747-759

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A New Possible Mechanism by Which Punicalagin Protects against Liver Injury Induced by Type 2 Diabetes Mellitus: Upregulation of Autophagy via the Akt/FoxO3a Signaling Pathway.

Zhang Y, Cao Y, Chen J, Qin H, Yang L
Journal of agricultural and food chemistry 2019 Dec 18;67(50):13948-13959

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Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets.

Xu S, Xu ZX, Yan S, Le J, Chen H, Ming L, Xu SG, Lin T
Experimental and therapeutic medicine 2019 Aug;18(2):1099-1106

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Formononetin inhibits colon carcinoma cell growth and invasion by microRNA‑149‑mediated EphB3 downregulation and inhibition of PI3K/AKT and STAT3 signaling pathways.

Wang AL, Li Y, Zhao Q, Fan LQ
Molecular medicine reports 2018 Jun;17(6):7721-7729

---

CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.

Sun X, Huang S, Wang X, Zhang X, Wang X
Oncotarget 2018 Jun 8;9(44):27574-27584

---

Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties.

Wang S, Xu M, Li X, Su X, Xiao X, Keating A, Zhao RC
Journal of hematology & oncology 2018 Jun 14;11(1):82

---

Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells.

Mou JJ, Peng J, Shi YY, Li N, Wang Y, Ke Y, Zhou YF, Zhou FX
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jul;103:729-737

---

SIRT3 is correlated with the malignancy of non-small cell lung cancer.

Xiong Y, Wang M, Zhao J, Wang L, Li X, Zhang Z, Jia L, Han Y
International journal of oncology 2017 Mar;50(3):903-910

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Dual Inhibition of PI3K/AKT and MEK/ERK Pathways Induces Synergistic Antitumor Effects in Diffuse Intrinsic Pontine Glioma Cells.

Wu YL, Maachani UB, Schweitzer M, Singh R, Wang M, Chang R, Souweidane MM
Translational oncology 2017 Apr;10(2):221-228

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Nuclear PTEN interferes with binding of Ku70 at double-strand breaks through post-translational poly(ADP-ribosyl)ation.

Guan J, Zhao Q, Mao W
Biochimica et biophysica acta 2016 Dec;1863(12):3106-3115

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 60203-1-Ig(AKT antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using AKT1 antibody 60203-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line