60203-1-Ig

antibody from Proteintech Group

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

60203-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#60203-1-Ig, RRID:AB_10919160

Product name

AKT1/3 antibody

Antibody type

Monoclonal

Description

KD/KO validated AKT1/3 antibody (Cat. #60203-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

3G5E11

Vial size

20ul, 150ul

References

Submitted references

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Babao Dan Reverses Multiple-Drug Resistance in Gastric Cancer Cells via Triggering Apoptosis and Autophagy and Inhibiting PI3K/AKT/mTOR Signaling.

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BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.

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The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway.

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Journal of neurochemistry 2021 May;157(3):467-478

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miR-320a/SP1 negative reciprocal interaction contributes to cell growth and invasion in colorectal cancer.

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RabGEF1 functions as an oncogene in U251 glioblastoma cells and is involved in regulating AKT and Erk pathways.

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Peng X, Hao M, Zhao Y, Cai Y, Chen X, Chen H, Zhang Y, Dong L, Liu X, Ding C, Liu W, Yang M, Luo Y
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The orphan nuclear receptor Nurr1 agonist amodiaquine mediates neuroprotective effects in 6-OHDA Parkinson's disease animal model by enhancing the phosphorylation of P38 mitogen-activated kinase but not PI3K/AKT signaling pathway.

Kambey PA, Chengcheng M, Xiaoxiao G, Abdulrahman AA, Kanwore K, Nadeem I, Jiao W, Gao D
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miR-31-5p Regulates 14-3-3 ɛ to Inhibit Prostate Cancer 22RV1 Cell Survival and Proliferation via PI3K/AKT/Bcl-2 Signaling Pathway.

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Proanthocyanidin B2 inhibits proliferation and induces apoptosis of osteosarcoma cells by suppressing the PI3K/AKT pathway.

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siPRDX2-elevated DNM3 inhibits the proliferation and metastasis of colon cancer cells via AKT signaling pathway.

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Fabrication of high-strength mecobalamin loaded aligned silk fibroin scaffolds for guiding neuronal orientation.

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Ren Z, Chen L, Wang Y, Wei X, Zeng S, Zheng Y, Gao C, Liu H
Journal of immunology (Baltimore, Md. : 1950) 2019 Feb 1;202(3):747-759

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A New Possible Mechanism by Which Punicalagin Protects against Liver Injury Induced by Type 2 Diabetes Mellitus: Upregulation of Autophagy via the Akt/FoxO3a Signaling Pathway.

Zhang Y, Cao Y, Chen J, Qin H, Yang L
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Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets.

Xu S, Xu ZX, Yan S, Le J, Chen H, Ming L, Xu SG, Lin T
Experimental and therapeutic medicine 2019 Aug;18(2):1099-1106

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Formononetin inhibits colon carcinoma cell growth and invasion by microRNA‑149‑mediated EphB3 downregulation and inhibition of PI3K/AKT and STAT3 signaling pathways.

Wang AL, Li Y, Zhao Q, Fan LQ
Molecular medicine reports 2018 Jun;17(6):7721-7729

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CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.

Sun X, Huang S, Wang X, Zhang X, Wang X
Oncotarget 2018 Jun 8;9(44):27574-27584

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Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties.

Wang S, Xu M, Li X, Su X, Xiao X, Keating A, Zhao RC
Journal of hematology & oncology 2018 Jun 14;11(1):82

---

Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells.

Mou JJ, Peng J, Shi YY, Li N, Wang Y, Ke Y, Zhou YF, Zhou FX
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jul;103:729-737

---

SIRT3 is correlated with the malignancy of non-small cell lung cancer.

Xiong Y, Wang M, Zhao J, Wang L, Li X, Zhang Z, Jia L, Han Y
International journal of oncology 2017 Mar;50(3):903-910

---

Dual Inhibition of PI3K/AKT and MEK/ERK Pathways Induces Synergistic Antitumor Effects in Diffuse Intrinsic Pontine Glioma Cells.

Wu YL, Maachani UB, Schweitzer M, Singh R, Wang M, Chang R, Souweidane MM
Translational oncology 2017 Apr;10(2):221-228

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Nuclear PTEN interferes with binding of Ku70 at double-strand breaks through post-translational poly(ADP-ribosyl)ation.

Guan J, Zhao Q, Mao W
Biochimica et biophysica acta 2016 Dec;1863(12):3106-3115

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 60203-1-Ig(AKT antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using AKT1 antibody 60203-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line