MA5-14898

Arjunan P, Meghil MM, Pi W, Xu J, Lang L, El-Awady A, Sullivan W, Rajendran M, Rabelo MS, Wang T, Tawfik OK, Kunde-Ramamoorthy G, Singh N, Muthusamy T, Susin C, Teng Y, Arce RM, Cutler CW
Scientific reports 2018 Nov 9;8(1):16607

Hsp90-targeted miRNA-liposomal formulation for systemic antitumor effect.

Pore SK, Choudhary A, Rathore B, Ganguly A, Sujitha P, Kumar CG, Agawane SB, Kumar JM, Scaria V, Pillai B, Banerjee R
Biomaterials 2013 Sep;34(28):6804-17

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Akt1 in extracts from Akt3 (lane 1) knock-out mouse embryonic fibroblasts (MEFs) and matched wild-type MEFs (lane 2) using Akt1 monoclonal antibody (Product # MA5-14898) (upper) or an Akt2 antibody (lower).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Akt1 in recombinant Akt1, Akt2 and Akt3 proteins using Akt1 monoclonal antibody (Product # MA5-14898) (upper) or an Akt (pan) monoclonal antibody (lower).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Chromatin immunoprecipitation analysis of Akt1 was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of an Atk1 monoclonal antibody (Product # MA5-14898). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Wogonin promotes the expression of p-Akt following TBI in the hippocampus through PI3K. (A) western blotting was performed on the levels of p-Akt and Akt in the hippocampal CA1 region of the sham group, TBI group, wogonin administration group (TBI + Wog), wogonin and LY294002 administration group (TBI + Wog + LY) and DMSO control group (TBI + DMSO) at 24 h after the operation. (B) The relative optical density of the p-Akt band was analyzed. (C) Representative immunofluorescence confocal images were shown to evaluate the colocalization of p-Akt (green) and NeuN (red). (D) The quantitative analysis of p-Akt-positive NeuN in the hippocampus. Scale bar, 50 u m. * P