MA5-14898

antibody from Invitrogen Antibodies

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14898

Provider

Invitrogen Antibodies

Product name

AKT1 Monoclonal Antibody (G.145.7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with Akt2 or Akt3.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

G.145.7

Vial size

100 µL

Concentration

35 µg/mL

Storage

-20°C

References

Submitted references

Protective role of wogonin following traumatic brain injury by reducing oxidative stress and apoptosis via the PI3K/Nrf2/HO‑1 pathway.

Feng Y, Ju Y, Yan Z, Ji M, Yang M, Wu Q, Wang L, Sun G
International journal of molecular medicine 2022 Apr;49(4)

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Oral Pathobiont Activates Anti-Apoptotic Pathway, Promoting both Immune Suppression and Oncogenic Cell Proliferation.

Arjunan P, Meghil MM, Pi W, Xu J, Lang L, El-Awady A, Sullivan W, Rajendran M, Rabelo MS, Wang T, Tawfik OK, Kunde-Ramamoorthy G, Singh N, Muthusamy T, Susin C, Teng Y, Arce RM, Cutler CW
Scientific reports 2018 Nov 9;8(1):16607

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Hsp90-targeted miRNA-liposomal formulation for systemic antitumor effect.

Pore SK, Choudhary A, Rathore B, Ganguly A, Sujitha P, Kumar CG, Agawane SB, Kumar JM, Scaria V, Pillai B, Banerjee R
Biomaterials 2013 Sep;34(28):6804-17

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Akt1 in extracts from Akt3 (lane 1) knock-out mouse embryonic fibroblasts (MEFs) and matched wild-type MEFs (lane 2) using Akt1 monoclonal antibody (Product # MA5-14898) (upper) or an Akt2 antibody (lower).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Akt1 in recombinant Akt1, Akt2 and Akt3 proteins using Akt1 monoclonal antibody (Product # MA5-14898) (upper) or an Akt (pan) monoclonal antibody (lower).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of Akt1 was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of an Atk1 monoclonal antibody (Product # MA5-14898). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Wogonin promotes the expression of p-Akt following TBI in the hippocampus through PI3K. (A) western blotting was performed on the levels of p-Akt and Akt in the hippocampal CA1 region of the sham group, TBI group, wogonin administration group (TBI + Wog), wogonin and LY294002 administration group (TBI + Wog + LY) and DMSO control group (TBI + DMSO) at 24 h after the operation. (B) The relative optical density of the p-Akt band was analyzed. (C) Representative immunofluorescence confocal images were shown to evaluate the colocalization of p-Akt (green) and NeuN (red). (D) The quantitative analysis of p-Akt-positive NeuN in the hippocampus. Scale bar, 50 u m. * P