PA5-29169

antibody from Invitrogen Antibodies

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29169

Provider

Invitrogen Antibodies

Product name

AKT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HA-AKT transfected HeLa cell, mouse liver. Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (84%), Xenopus laevis (90%), Cat (98%), Pig (96%), Chicken (96%), Sheep (96%), Rhesus Monkey (99%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.47 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.

Liu J, Li C, Wang Q, Hu H, Li C, Qian J
Journal of healthcare engineering 2022;2022:1473597

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Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain.

Chia K, Mazzolini J, Mione M, Sieger D
eLife 2018 Feb 21;7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AKT1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AKT1 Polyclonal Antibody (Product # PA5-29169) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AKT1 Polyclonal Antibody (Product # PA5-29169). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. AKT1 Polyclonal Antibody (Product # PA5-29169) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AKT1 Polyclonal Antibody (Product # PA5-29169). Sample (20 µg of whole cell lysate). Lane A: HeLa cell lysate. Lane B: HeLa cell transfect with AKT . 7.5% SDS PAGE. AKT1 Polyclonal Antibody (Product # PA5-29169) diluted at 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AKT1 Polyclonal Antibody (Product # PA5-29169). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 Polyclonal Antibody (Product # PA5-29169) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of AKT1 showing staining in the cytoplasm of MCF-7 cells. MCF-7 cells were fixed in ice-cold MeOH for 5 min and stained using an AKT1 polyclonal antibody (Product # PA5-29169) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1 (Product # PA5-29169) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 µg whole cell extract of Akt1-transfected 293T cells. A. 30 µg whole cell extract of Akt1-protein expressing 293T cell. B. Control with 3 µg of preimmune Rabbit IgG. C. Immunoprecipitation of Akt1 protein by 3 µg Akt1 antibody (Product # PA5-29169). 10 % SDS-PAGE. The immunoprecipitated Akt1 protein was detected by Akt1 antibody (Product # PA5-29169) diluted at 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 µg whole cell extract of Akt1-transfected 293T cells. A. 30 µg whole cell extract of Akt1-protein expressing 293T cell. B. Control with 3 µg of preimmune Rabbit IgG. C. Immunoprecipitation of Akt1 protein by 3 µg Akt1 antibody (Product # PA5-29169). 10 % SDS-PAGE. The immunoprecipitated Akt1 protein was detected by Akt1 antibody (Product # PA5-29169) diluted at 1:5000.