Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- SM7001P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM7001P, RRID:AB_972605
- Product name
- anti AKT1 / PKB pSer473
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 104A282
- Vial size
- 0.1 mg
- Concentration
- 0.5 ml/ml
Submitted references Critical analysis of simultaneous blockage of histone deacetylase and multiple receptor tyrosine kinase in the treatment of prostate cancer.
Inhibitory effects of the HDAC inhibitor valproic acid on prostate cancer growth are enhanced by simultaneous application of the mTOR inhibitor RAD001.
Curcumin inhibits the Sonic Hedgehog signaling pathway and triggers apoptosis in medulloblastoma cells.
Combining the receptor tyrosine kinase inhibitor AEE788 and the mammalian target of rapamycin (mTOR) inhibitor RAD001 strongly inhibits adhesion and growth of renal cell carcinoma cells.
Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis.
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Deguelin, an Akt inhibitor, suppresses IkappaBalpha kinase activation leading to suppression of NF-kappaB-regulated gene expression, potentiation of apoptosis, and inhibition of cellular invasion.
Wedel S, Hudak L, Seibel JM, Juengel E, Oppermann E, Haferkamp A, Blaheta RA
The Prostate 2011 May 15;71(7):722-35
The Prostate 2011 May 15;71(7):722-35
Inhibitory effects of the HDAC inhibitor valproic acid on prostate cancer growth are enhanced by simultaneous application of the mTOR inhibitor RAD001.
Wedel S, Hudak L, Seibel JM, Juengel E, Tsaur I, Wiesner C, Haferkamp A, Blaheta RA
Life sciences 2011 Feb 28;88(9-10):418-24
Life sciences 2011 Feb 28;88(9-10):418-24
Curcumin inhibits the Sonic Hedgehog signaling pathway and triggers apoptosis in medulloblastoma cells.
Elamin MH, Shinwari Z, Hendrayani SF, Al-Hindi H, Al-Shail E, Khafaga Y, Al-Kofide A, Aboussekhra A
Molecular carcinogenesis 2010 Mar;49(3):302-14
Molecular carcinogenesis 2010 Mar;49(3):302-14
Combining the receptor tyrosine kinase inhibitor AEE788 and the mammalian target of rapamycin (mTOR) inhibitor RAD001 strongly inhibits adhesion and growth of renal cell carcinoma cells.
Juengel E, Engler J, Natsheh I, Jones J, Mickuckyte A, Hudak L, Jonas D, Blaheta RA
BMC cancer 2009 May 27;9:161
BMC cancer 2009 May 27;9:161
Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis.
Shakibaei M, John T, Schulze-Tanzil G, Lehmann I, Mobasheri A
Biochemical pharmacology 2007 May 1;73(9):1434-45
Biochemical pharmacology 2007 May 1;73(9):1434-45
Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
Luty WH, Rodeberg D, Parness J, Vyas YM
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 15;179(2):819-29
Deguelin, an Akt inhibitor, suppresses IkappaBalpha kinase activation leading to suppression of NF-kappaB-regulated gene expression, potentiation of apoptosis, and inhibition of cellular invasion.
Nair AS, Shishodia S, Ahn KS, Kunnumakkara AB, Sethi G, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5612-22
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5612-22
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Akt1 using IMG-187 at 2 µg/ml dilution against 10 ug/lane of untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of phospho AKT using phospho AKT antibody at 2 µg/ml against untreated (Lane 1) and PDGF treated (Lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test.
