AMRe83751

antibody from EnkiLife Biotech Co., Ltd.

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMRe83751

Provider

EnkiLife Biotech Co., Ltd.

Product name

AKT1/2/3 Rabbit Monoclonal Antibody

Antibody type

Recombinant

Description

Affinity Purification

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

Monoclonal

Vial size

100 µl

Concentration

0.34 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Western blot analysis of AKT1 + AKT2 + AKT3 expression in (1) Hela cell lysate;(2) A549 cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Immunoprecipitation

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)

Flow cytometry

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Supportive validation

Submitted by

EnkiLife Biotech Co., Ltd. (provider)

Main image

Experimental details

Immunofluorescent analysis using the Antibody at 1:50 dilution.