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- Product number
- GTX128414 - Provider product page
- Provider
- GeneTex
- Product name
- AKT (phospho Ser473) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system.
Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells.
MiR-338-3p inhibits TNF-α-induced lipogenesis in human sebocytes.
Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
The effects of artocarpin on wound healing: in vitro and in vivo studies.
N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms.
Electroacupuncture Promotes Recovery of Motor Function and Reduces Dopaminergic Neuron Degeneration in Rodent Models of Parkinson's Disease.
The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity.
The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells.
Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation.
Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Constitutively active Akt1 cooperates with KRas(G12D) to accelerate in vivo pancreatic tumor onset and progression.
Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A.
Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production.
Li HY, Leu YL, Wu YC, Wang SH
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901
Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
Ko PH, Shen YC, Murugan K, Huang CW, Sivakumar G, Pal P, Liao CC, Luo KS, Chuang EY, Tsai MH, Lai LC
Scientific reports 2019 Feb 14;9(1):2147
Scientific reports 2019 Feb 14;9(1):2147
Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system.
Viedma-Rodríguez R, Martínez-Hernández MG, Flores-López LA, Baiza-Gutman LA
Molecular and cellular biochemistry 2018 Jan;437(1-2):65-80
Molecular and cellular biochemistry 2018 Jan;437(1-2):65-80
Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells.
Wu PY, You YJ, Liu YJ, Hou CW, Wu CS, Wen KC, Lin CY, Chiang HM
International journal of molecular sciences 2018 Apr 7;19(4)
International journal of molecular sciences 2018 Apr 7;19(4)
MiR-338-3p inhibits TNF-α-induced lipogenesis in human sebocytes.
Liu J, Cao L, Feng Y, Li Y, Li T
Biotechnology letters 2017 Sep;39(9):1343-1349
Biotechnology letters 2017 Sep;39(9):1343-1349
Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Wang JP, Hsieh CH, Liu CY, Lin KH, Wu PT, Chen KM, Fang K
Oncology letters 2017 Sep;14(3):3503-3509
Oncology letters 2017 Sep;14(3):3503-3509
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Ni YL, Hsieh CH, Kim SH, Wang JP, Su CL, Yao CF, Fang K
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Liu J, Chen W, Zhang H, Liu T, Zhao L
Oncology letters 2017 Nov;14(5):5711-5718
Oncology letters 2017 Nov;14(5):5711-5718
The effects of artocarpin on wound healing: in vitro and in vivo studies.
Yeh CJ, Chen CC, Leu YL, Lin MW, Chiu MM, Wang SH
Scientific reports 2017 Nov 15;7(1):15599
Scientific reports 2017 Nov 15;7(1):15599
N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms.
Kuo YH, Chen CC, Wu PY, Wu CS, Sung PJ, Lin CY, Chiang HM
BMC complementary and alternative medicine 2017 Jan 23;17(1):71
BMC complementary and alternative medicine 2017 Jan 23;17(1):71
Electroacupuncture Promotes Recovery of Motor Function and Reduces Dopaminergic Neuron Degeneration in Rodent Models of Parkinson's Disease.
Lin JG, Chen CJ, Yang HB, Chen YH, Hung SY
International journal of molecular sciences 2017 Aug 24;18(9)
International journal of molecular sciences 2017 Aug 24;18(9)
The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity.
Chen PH, Shen WL, Shih CM, Ho KH, Cheng CH, Lin CW, Lee CC, Liu AJ, Chen KC
Neuropharmacology 2017 Apr;116:300-314
Neuropharmacology 2017 Apr;116:300-314
The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Chen CC, Lin MW, Liang CJ, Wang SH
PloS one 2016;11(7):e0158662
PloS one 2016;11(7):e0158662
Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Chen CC, Liang CJ, Leu YL, Chen YL, Wang SH
PloS one 2016;11(12):e0168092
PloS one 2016;11(12):e0168092
The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Chiu WC, Lee YC, Su YH, Wang YY, Tsai CH, Hou YA, Wang CH, Huang YF, Huang CJ, Chou SH, Hsieh PW, Yuan SF
PloS one 2016;11(11):e0166453
PloS one 2016;11(11):e0166453
A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells.
Hsieh CH, Lee LC, Leong WY, Yang TC, Yao CF, Fang K
Drug design, development and therapy 2016;10:2947-2957
Drug design, development and therapy 2016;10:2947-2957
Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation.
Vallejo-Flores G, Torres J, Sandoval-Montes C, Arévalo-Romero H, Meza I, Camorlinga-Ponce M, Torres-Morales J, Chávez-Rueda AK, Legorreta-Haquet MV, Fuentes-Pananá EM
BioMed research international 2015;2015:761501
BioMed research international 2015;2015:761501
Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Chen CY, Lin YS, Chen CL, Chao PZ, Chiou JF, Kuo CC, Lee FP, Lin YF, Sung YH, Lin YT, Li CF, Chen YJ, Chen CH
Oncotarget 2015 Sep 29;6(29):26946-59
Oncotarget 2015 Sep 29;6(29):26946-59
Constitutively active Akt1 cooperates with KRas(G12D) to accelerate in vivo pancreatic tumor onset and progression.
Albury TM, Pandey V, Gitto SB, Dominguez L, Spinel LP, Talarchek J, Klein-Szanto AJ, Testa JR, Altomare DA
Neoplasia (New York, N.Y.) 2015 Feb;17(2):175-82
Neoplasia (New York, N.Y.) 2015 Feb;17(2):175-82
Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A.
Chao TT, Wang CY, Chen YL, Lai CC, Chang FY, Tsai YT, Chao CH, Shiau CW, Huang YC, Yu CJ, Chen KF
Oncotarget 2015 Feb 10;6(4):2164-79
Oncotarget 2015 Feb 10;6(4):2164-79
Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production.
Sun W, Depping R, Jelkmann W
Cell death & disease 2014 Jan 23;5:e1020
Cell death & disease 2014 Jan 23;5:e1020
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Akt1/2/3 (phospho Ser473/474/472) antibody detects Akt1/2/3 (phospho Ser473/474/472) protein by Western blot analysis.A. 30 ?g MCF7 whole cell lysate/extract (untreated)B. 30 ?g MCF7 whole cell lysate/extract (500nM PMA treatment for 1hr)C. 30 ?g MCF7 whole cell lysate/extract (500nM PMA treatment for 2hr)D. 30 ?g MCF7 whole cell lysate/extract (500nM PMA treatment for 4hr)E. 30 ?g MCF7 whole cell lysate/extract (500nM PMA treatment for 6hr)7.5 % SDS-PAGEAkt1/2/3 (phospho Ser473/474/472) antibody (GTX128414) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCF-7 cells were untreated or treated with 500 nM PMA for 1, 2, 4 and 6 hrs. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) NIH-3T3 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HUVEC whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Akt1/2/3 (phospho Ser473/474/472) protein from 293T whole cell extracts using 5 £gg of Akt1/2/3 (phospho Ser473/474/472) antibody (GTX128414).Western blot analysis was performed using Akt1/2/3 (phospho Ser473/474/472) antibody (GTX128414).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
