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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ABIN1504173 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 'NL50
- Vial size
- 0.1 mL
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- antibodies-online (provider)
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- Experimental details
- Figure 1. Western Blot Analysis (Representative image from a previous lot). Lysates from untreated (Lanes 1 and 3) and PDGF treated (Lanes 2 and 4) 3T3/NIH cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-Akt1/PKB? (Thr308) (1/2,000 dilution from a previous lot) (Lanes 1 and 2) or total anti-Akt1/PKB? (1/1,000 dilution from a previous lot) (Lanes 3 and 4).Proteins were visualized using a Goat anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated Akt1/PKB? (~60 kDa).
