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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- ABIN955115 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-T-Box 6 (TBX6) (AA 150-180), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 150-180 amino acids from the Central region of human TBX6
- Description
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 150-180,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The association analysis of TBX6 polymorphism with susceptibility to congenital scoliosis in a Chinese Han population.
A missense T (Brachyury) mutation contributes to vertebral malformations.
Transcriptional repression by the T-box proteins Tbx18 and Tbx15 depends on Groucho corepressors.
The human TBX6 gene: cloning and assignment to chromosome 16p11.2.
Identification, mapping, and phylogenomic analysis of four new human members of the T-box gene family: EOMES, TBX6, TBX18, and TBX19.
Fei Q, Wu Z, Wang H, Zhou X, Wang N, Ding Y, Wang Y, Qiu G
Spine 2010 Apr 20;35(9):983-8
Spine 2010 Apr 20;35(9):983-8
A missense T (Brachyury) mutation contributes to vertebral malformations.
Ghebranious N, Blank RD, Raggio CL, Staubli J, McPherson E, Ivacic L, Rasmussen K, Jacobsen FS, Faciszewski T, Burmester JK, Pauli RM, Boachie-Adjei O, Glurich I, Giampietro PF
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Oct;23(10):1576-83
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Oct;23(10):1576-83
Transcriptional repression by the T-box proteins Tbx18 and Tbx15 depends on Groucho corepressors.
Farin HF, Bussen M, Schmidt MK, Singh MK, Schuster-Gossler K, Kispert A
The Journal of biological chemistry 2007 Aug 31;282(35):25748-59
The Journal of biological chemistry 2007 Aug 31;282(35):25748-59
The human TBX6 gene: cloning and assignment to chromosome 16p11.2.
Papapetrou C, Putt W, Fox M, Edwards YH
Genomics 1999 Jan 15;55(2):238-41
Genomics 1999 Jan 15;55(2):238-41
Identification, mapping, and phylogenomic analysis of four new human members of the T-box gene family: EOMES, TBX6, TBX18, and TBX19.
Yi CH, Terrett JA, Li QY, Ellington K, Packham EA, Armstrong-Buisseret L, McClure P, Slingsby T, Brook JD
Genomics 1999 Jan 1;55(1):10-20
Genomics 1999 Jan 1;55(1):10-20
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- TBX6 Antibody (Center W158) (Cat. #AP54187PU-N) western blot analysis in mouse liver tissue and Hela cell line lysates (35μg/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- TBX6 Antibody (Center W158) (Cat. #AP54187PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
