11058-1-AP

antibody from Proteintech Group

Targeting: NONO NMT55, NRB54, P54, P54NRB, PPP1R114

Provider product page for 11058-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [38]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 11058-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11058-1-AP, RRID:AB_2152167
Product name: NONO antibody
Antibody type: Polyclonal
Description: KD/KO validated NONO antibody (Cat. #11058-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NONO protein structure - 11058-1-AP shown in red.

Submitted references Combating Atherosclerosis with Chirality/Phase Dual-Engineered Nanozyme Featuring Microenvironment-Programmed Senolytic and Senomorphic Actions.

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Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human.

Huang W, Xiong T, Zhao Y, Heng J, Han G, Wang P, Zhao Z, Shi M, Li J, Wang J, Wu Y, Liu F, Xi JJ, Wang Y, Zhang QC
Nature genetics 2024 Jan;56(1):124-135

Gankyrin-mediated interaction between cancer cells and tumor-associated macrophages facilitates prostate cancer progression and androgen deprivation therapy resistance.

Peng G, Wang C, Wang H, Qu M, Dong K, Yu Y, Jiang Y, Gan S, Gao X
Oncoimmunology 2023;12(1):2173422

LncRNA Neat1 targets NonO and miR-128-3p to promote antigen-specific Th17 cell responses and autoimmune inflammation.

Chen S, Wang J, Zhang K, Ma B, Li X, Wei R, Nian H
Cell death & disease 2023 Sep 16;14(9):610

PICH Activates Cyclin A1 Transcription to Drive S-Phase Progression and Chemoresistance in Gastric Cancer.

Ye H, Shi W, Yang J, Wang L, Jiang X, Zhao H, Qin L, Qin J, Li L, Cai W, Guan J, Yang H, Zhou H, Yu Z, Sun H, Jiao Z
Cancer research 2023 Nov 15;83(22):3767-3782

K235 acetylation couples with PSPC1 to regulate the m(6)A demethylation activity of ALKBH5 and tumorigenesis.

Zhang XL, Chen XH, Xu B, Chen M, Zhu S, Meng N, Wang JZ, Zhu H, Chen D, Liu JB, Yan GR
Nature communications 2023 Jun 27;14(1):3815

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges.

Miyagi T, Ueda K, Sugimoto M, Yagi T, Ito D, Yamazaki R, Narumi S, Hayamizu Y, Uji-I H, Kuroda M, Kanekura K
iScience 2023 Jun 16;26(6):106957

The KDET Motif in the Intracellular Domain of the Cell Adhesion Molecule L1 Interacts with Several Nuclear, Cytoplasmic, and Mitochondrial Proteins Essential for Neuronal Functions.

Kleene R, Loers G, Schachner M
International journal of molecular sciences 2023 Jan 4;24(2)

The Interactions of the 70 kDa Fragment of Cell Adhesion Molecule L1 with Topoisomerase 1, Peroxisome Proliferator-Activated Receptor γ and NADH Dehydrogenase (Ubiquinone) Flavoprotein 2 Are Involved in Gene Expression and Neuronal L1-Dependent Functions.

Loers G, Kleene R, Bork U, Schachner M
International journal of molecular sciences 2023 Jan 20;24(3)

The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA.

Hou YR, Diao LT, Hu YX, Zhang QQ, Lv G, Tao S, Xu WY, Xie SJ, Zhang Q, Xiao ZD
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Aug;10(23):e2301983

LncRNA NEAT1 Potentiates SREBP2 Activity to Promote Inflammatory Macrophage Activation and Limit Hantaan Virus Propagation.

Yang Y, Li M, Ma Y, Ye W, Si Y, Zheng X, Liu H, Cheng L, Zhang L, Zhang H, Zhang X, Lei Y, Shen L, Zhang F, Ma H
Frontiers in microbiology 2022;13:849020

Identification and characterization of a special type of subnuclear structure: AGGF1-coated paraspeckles.

Zhao J, Xie W, Yang Z, Zhao M, Ke T, Xu C, Li H, Chen Q, Wang QK
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Jun;36(6):e22366

Stabilization of SAMHD1 by NONO is crucial for Ara-C resistance in AML.

Zhang F, Sun J, Tang X, Liang Y, Jiao Q, Yu B, Dai Z, Yuan X, Li J, Yan J, Zhang Z, Fan S, Wang M, Hu H, Zhang C, Lv XB
Cell death & disease 2022 Jul 8;13(7):590

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Zhang S, Huang W, Ren L, Ju X, Gong M, Rao J, Sun L, Li P, Ding Q, Wang J, Zhang QC
Cell research 2022 Jan;32(1):9-23

Condensates induced by transcription inhibition localize active chromatin to nucleoli.

Yasuhara T, Xing YH, Bauer NC, Lee L, Dong R, Yadav T, Soberman RJ, Rivera MN, Zou L
Molecular cell 2022 Aug 4;82(15):2738-2753.e6

Circular RNA circTmem241 drives group III innate lymphoid cell differentiation via initiation of Elk3 transcription.

Liu N, He J, Fan D, Gu Y, Wang J, Li H, Zhu X, Du Y, Tian Y, Liu B, Fan Z
Nature communications 2022 Aug 11;13(1):4711

Identification of Key Transcription Factors and Immune Infiltration Patterns Associated With Breast Cancer Prognosis Using WGCNA and Cox Regression Analysis.

Yin X, Liu J, Wang X, Yang T, Li G, Shang Y, Teng X, Yu H, Wang S, Huang W
Frontiers in oncology 2021;11:742792

AC016745.3 Regulates the Transcription of AR Target Genes by Antagonizing NONO.

Lu Y, Wan X, Huang W, Zhang L, Luo J, Li D, Huang Y, Li Y, Xu Y
Life (Basel, Switzerland) 2021 Nov 9;11(11)

Expression of Long Non-coding RNA RGD1566344 in the Brain Cortex of Male Mice After Focal Cerebral Ischemia-Reperfusion and the Neuroprotective Effect of a Non-coding RNA RGD1566344 Inhibitor.

Zhang J, Rui Y, Gao M, Wang L, Yan BC
Cellular and molecular neurobiology 2021 May;41(4):705-716

Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling.

Cai D, Han JJ
Nature aging 2021 May;1(5):438-453

Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.

Zhang F, Tang X, Fan S, Liu X, Sun J, Ju C, Liang Y, Liu R, Zhou R, Yu B, Zhang C, Zhang Z, Kang T, Huang G, Lv XB
Oncogene 2021 Jun;40(24):4137-4150

OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.

Cui Y, Xie R, Zhang X, Liu Y, Hu Y, Li Y, Liu X, Yu X, Wu C
Cell death & disease 2021 Jun 16;12(7):622

The regulation of NONO by USP11 via deubiquitination is linked to the proliferation of melanoma cells.

Feng P, Li L, Dai J, Zhou L, Liu J, Zhao J, Li X, Ling N, Qiu S, Zhang L, Xie T, Chen Y, Donovan MJ, Peng T, Song J, Ye M
Journal of cellular and molecular medicine 2021 Feb;25(3):1507-1517

The transcribed ultraconserved element uc.51 promotes the proliferation and metastasis of breast cancer by stabilizing NONO.

Shi X, Huang X, Chen R, Li Y, Xu Y, Zhang W, Zhu Q, Zha X, Wang J
Clinical & experimental metastasis 2021 Dec;38(6):551-571

Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer.

Qin Y, Chen W, Jiang G, Zhou L, Yang X, Li H, He X, Wang HL, Zhou YB, Huang S, Liu S
Science advances 2020 Feb;6(8):eaaw9960

Long non-coding RNA SSTR5-AS1 facilitates gemcitabine resistance via stabilizing NONO in gallbladder carcinoma.

Xue Z, Yang B, Xu Q, Zhu X, Qin G
Biochemical and biophysical research communications 2020 Feb 19;522(4):952-959

Splicing Regulator p54(nrb) /Non-POU Domain-Containing Octamer-Binding Protein Enhances Carcinogenesis Through Oncogenic Isoform Switch of MYC Box-Dependent Interacting Protein 1 in Hepatocellular Carcinoma.

Hu Z, Dong L, Li S, Li Z, Qiao Y, Li Y, Ding J, Chen Z, Wu Y, Wang Z, Huang S, Gao Q, Zhao Y, He X
Hepatology (Baltimore, Md.) 2020 Aug;72(2):548-568

Long non-coding RNA LINC-PINT attenuates paclitaxel resistance in triple-negative breast cancer cells via targeting the RNA-binding protein NONO.

Chen J, Zhu M, Zou L, Xia J, Huang J, Deng Q, Xu R
Acta biochimica et biophysica Sinica 2020 Aug 5;52(8):801-809

Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.

Zhang J, Chen Y, Gao M, Wang Z, Liu R, Xia T, Liu S
ACS nano 2019 Feb 26;13(2):2050-2061

Characterization of telomeric repeat-containing RNA (TERRA) localization and protein interactions in primordial germ cells of the mouse†.

Brieño-Enríquez MA, Moak SL, Abud-Flores A, Cohen PE
Biology of reproduction 2019 Apr 1;100(4):950-962

A variant of death-receptor 3 associated with rheumatoid arthritis interferes with apoptosis-induction of T cell.

Hashiramoto A, Konishi Y, Murayama K, Kawasaki H, Yoshida K, Tsumiyama K, Tanaka K, Mizuhara M, Shiotsuki T, Kitamura H, Komai K, Kimura T, Yagita H, Shiozawa K, Shiozawa S
The Journal of biological chemistry 2018 Feb 9;293(6):1933-1943

The Long Noncoding RNA NEAT1 Exerts Antihantaviral Effects by Acting as Positive Feedback for RIG-I Signaling.

Ma H, Han P, Ye W, Chen H, Zheng X, Cheng L, Zhang L, Yu L, Wu X, Xu Z, Lei Y, Zhang F
Journal of virology 2017 May 1;91(9)

Synergistic upregulation of NONO and PSPC1 regulates Sertoli cell response to MEHP via modulation of ALDH1A1 signaling.

Dong BW, Jin XH, Yan CY, Yang T, Cai GQ, Lu J
FEBS letters 2017 Mar;591(6):914-923

Distinct Protein Expression Profiles of Solid-Pseudopapillary Neoplasms of the Pancreas.

Park M, Lim JS, Lee HJ, Na K, Lee MJ, Kang CM, Paik YK, Kim H
Journal of proteome research 2015 Aug 7;14(8):3007-14

The transcription-splicing protein NonO/p54nrb and three NonO-interacting proteins bind to distal enhancer region and augment rhodopsin expression.

Yadav SP, Hao H, Yang HJ, Kautzmann MA, Brooks M, Nellissery J, Klocke B, Seifert M, Swaroop A
Human molecular genetics 2014 Apr 15;23(8):2132-44

14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage.

Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X
PloS one 2013;8(3):e55268

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma.

Yang X, Zou P, Yao J, Yun D, Bao H, Du R, Long J, Chen X
Journal of proteome research 2010 Mar 5;9(3):1402-15

Biotin tagging coupled with amino acid-coded mass tagging for efficient and precise screening of interaction proteome in mammalian cells.

He YF, Bao HM, Xiao XF, Zuo S, Du RY, Tang SW, Yang PY, Chen X
Proteomics 2009 Dec;9(24):5414-24

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP(NONO antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using NONO antibody 11058-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 11058-1-AP(NONO antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue