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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX19407 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX19407, RRID:AB_423570
- Product name
- p54nrb antibody [78-1 C]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Rabbit
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunohistochemistry was performed on normal deparaffinized human Kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:500 with or without nmt55 / p54nrb antibody [78-1 C] overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
