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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- PA5-18514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NONO Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HEK293 overexpressing Human NONO using Product # PA5-18514, mock transfection as a control in first lane.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Mouse brain lysate using Product # PA5-18514 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HEK293 overexpressing Human NONO using Product # PA5-18514, mock transfection as a control in first lane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NONO in paraffin embedded human spleen using a NONO polyclonal antibody (Product #PA5-18514) at a concentration of 3.75 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NONO in paraffin-embedded Human testis using a NONO polyclonal antibody (Product #PA5-18514) at a dilution of 3.75µg/ml. Steamed antigen retrieval was performed using citrate buffer at a pH of 6. The sample was counterstained with Alkaline Phosphatase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NONO in paraffin-embedded Human spleen using a NONO polyclonal antibody (Product #PA5-18514) at a dilution of 3.75µg/ml. Steamed antigen retrieval was performed using citrate buffer at a pH of 6. The sample was counterstained with Alkaline Phosphatase.
