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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 102-PA26S - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- CCM-2
- Antibody type
- Polyclonal
- Antigen
- Recombinant human CCM2
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
MGSSHHHHHHSSGLVPRGSHMEEEGKKGKKPGIVS
PFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLSLP
ERVEPDRLLSDYIEKEVKYLGQLTSIPGYLNPSSR
TEILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYNV
KLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVL
KTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPV
EACCLVILAAESKVAAEELCCLLGQVFQVVYTEST
IDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKET
YEVEASTFCFPESVDVGGASPHSKTISESELSASA
TELLQDYMLTLRTKLSSQEIQQFAALLHEYRNGAS
IHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQHF
ENFLETIGVKDGRGIITDSFGRHRRALSTTSSSTT
NGNRATGSSDDRSAPSEGDEWDRMISDISSDIEAL
GCSMDQDSA- Antibody clone number
- Rabbit IG
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis of anti-human CCM-2. The experiment was performed by Elisabetta Dejana’s group, IFOM-IEO-Campus, Milan Italy
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of human foreskin (cryo-section of unfixed tissue) with anti-human CCM2 (K5546; dilution 1:50) [Cat# 102-PA26]. A) Note specific staining in the wall of microvessels. B) Negative control of a consecutive section. Note non-specific fluorescence in elastic fibres in the adventitia of an arteriol. Nuclei counter-stained with Dapi (blue). Specimen provided by Prof. Dr. J. Wilting, Goettingen. The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
- Sample type
- Human Foreskin
