16840-1-AP

antibody from Proteintech Group

Targeting: USP13 IsoT-3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16840-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16840-1-AP, RRID:AB_2214569

Product name

USP13 antibody

Antibody type

Polyclonal

Description

KD/KO validated USP13 antibody (Cat. #16840-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

USP13 ameliorates diabetic cardiomyopathy via deubiquitinating NLRP3 and inhibiting pyroptosis in cardiomyocytes.

Xu D, Han J, Ye B, Lin L, Chen J, Jin Y, Zhao Y, Wang Y, Shan P, Liang G
Cell death and differentiation 2026 May;33(5):914-929

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Bushen Huoxue formula component β-Estradiol 3-acetate treats osteoarthritis through enhancing the TLR4 ubiquitination.

Liu X, Wang S, Kuang C, Deng Y, Yuan S, Zou J
The Journal of steroid biochemistry and molecular biology 2026 Jan;255:106862

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USP13 ameliorates myocardial infarction injury by inhibiting ferroptosis via stabilizing ALDOA.

Li P, Chen D, Zhang C, Gong Q, Yidilisi A, Fang J, Zhang Y, Jin J, Huang J, Jiang J
Redox biology 2026 Feb;89:103995

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Non-classic deubiquitinase USP13 inhibits bladder cancer metastasis through destabilizing cytoplasmic KDM3A.

Hu H, Zhan X, Xiong Y, Yuan R, Du Y, Dong Q, Li S, Guo B, Li Z, Feng J, Xiong S, Xiong J, Li D, Fu B, Xu S, Guo J
Oncogene 2026 Apr;45(15):1324-1337

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USP13 stabilizes SOCS1 to reverse αPD-1 resistance in MSI-H colorectal cancer.

Zheng Z, Ni Y, Wu M, Ding L, Li H, Zhang N, Jin X, Zheng H, Jiang B, Wang S, Xu W, Wang Q, Fu J
Oncogene 2026 Apr 18;

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USP13 Suppresses Colorectal Cancer Angiogenesis by Downregulating VEGFA Expression through Inhibition of the PTEN-AKT Pathway.

Xu GZ, Guan HY, Guo YG, Zhang YR, Pan JH, Luo S, Ding H, Pan Y, Yao Q
Oncology research 2025;33(8):1947-1967

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USP13 mediates resistance to Ibrutinib in diffuse large B-cell lymphoma via augmenting FHL1 stabilization.

Tao Y, Yin H, Shen R, He S, Miao X
American journal of cancer research 2025;15(7):2932-2948

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Intestinal Epithelial-Derived USP13 Alleviates Colonic Inflammation by Suppressing GRP78-mediated Endoplasmic Reticulum Stress.

Qian C, Hu C, Xu Y, Xu W, Wang Z, Gan W, Tang W, Huang L, Samorodov AV, Wang Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Oct;12(38):e00741

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USP13 promotes colorectal cancer progression by deubiquitinating and stabilizing HIF-1α.

Gu B, Chang SY, Li KK, Wang BQ, Ke SB, Zhang XD, Du RL
Cell communication and signaling : CCS 2025 Nov 21;23(1):502

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Deubiquitinase USP13 alleviates doxorubicin-induced cardiotoxicity through promoting the autophagy-mediated degradation of STING.

Lin L, Han J, Xu D, Fang Z, Ye B, Qian J, Han X, Min J, Long X, Wu G, Liang G
Acta pharmaceutica Sinica. B 2025 May;15(5):2545-2558

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USP13 inhibition exacerbates mitochondrial dysfunction and acute kidney injury by acting on MCL-1.

Wang Q, Cao S, Sun Z, Zhu W, Sun L, Li Y, Luo D, Huang S, Zhang Y, Xia W, Zhang A, Jia Z
Biochimica et biophysica acta. Molecular basis of disease 2025 Mar;1871(3):167599

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USP13 promotes hepatic stellate cells activation and aggravates liver fibrosis through deubiquitinating SMAD3.

Wang A, Dong H, Cui Y, Zhu W, Ye J, Jin L, Xiong Y, Zhang Q, Lin M, Han J, Fang Z, Hu X, Luo W, Liang G
Hepatology international 2025 Jul 28;

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The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein.

Yang L, An X, Yang S, Lin X, Chen Z, Xue Q, Chen X, Wang Y, Yan D, Chen S, Fan Y, Tang D, Yu W, Liu J, Chen X
Cell death & disease 2025 Jul 26;16(1):564

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Cardiomyocyte-derived USP13 protects hearts from hypertrophy via deubiquitinating and stabilizing STAT1 in male mice.

Han J, Lin L, Fang Z, Xu D, Wang L, Ye B, Han X, Long X, Min J, Wu G, Liang G, Wang Y
Nature communications 2025 Jul 1;16(1):5927

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Spautin-1 alleviates depression-like behaviors in mice after traumatic brain injury by inhibiting MMP3 expression in microglia in the Hippocampal CA1 region.

Sun JK, Ma XY, Liu LT, Hui JY, Fu Y, Ge JL, Zhang YM, Xi H, Zhang DX, Zhang LM, Miao HT
Experimental neurology 2025 Dec;394:115462

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Hypoxia-induced USP13 expression drives ferroptosis resistance and tumor immune evasion in hepatocellular carcinoma through the stabilization of ACLY.

Hu K, Li J, Chen K, Mi X, Pan Y, Tang J, Cao J, Zhong X
Cell death discovery 2025 Dec 2;12(1):28

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USP13-Mediated selective autophagy of eIF5B promotes pathological cardiac hypertrophy.

Ye Y, Xu J, Jin C, Li X, Wu X, Zhou C, Gao L, Zhong Z, Li X, Chen S, Wei Y, Peng S, Liu S
Cellular and molecular life sciences : CMLS 2025 Dec 16;83(1):36

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Spautin-1 inhibits the growth of diffuse large B-cell lymphoma by inducing mitochondrial damage-mediated PANoptosis and anti-tumor immunity.

Wu J, Deng Y, Gao Y, Wei B
Cancer immunology, immunotherapy : CII 2025 Aug 25;74(9):293

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Bruceine A Inhibits Cell Proliferation by Targeting the USP13/PARP1 Signalling Pathway in Multiple Myeloma.

Guo M, Meng H, Sun Y, Zhou L, Hu T, Yu T, Bai H, Zhang Y, Gu C, Yang Y
Basic & clinical pharmacology & toxicology 2025 Apr;136(5):e70027

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Unveiling the Pharmacological Role of Human Deubiquitinating Enzymes in Temozolomide Response of Glioblastoma Cells.

Yang C, Li Y, Wu Q, Tang J, Chen M, Zhang B, Li B, Qin Y, Huang G, Zhang Y, Zhi F, Liu K
Cell biochemistry and biophysics 2024 Sep;82(3):2183-2193

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CaMKII Exacerbates Doxorubicin-Induced Cardiotoxicity by Promoting Ubiquitination Through USP10 Inhibition.

Yang Y, Wang Z, Wang N, Yang J, Yang L
Cancer medicine 2024 Nov;13(21):e70286

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Molecular Regulation of Transforming Growth Factor-β1-induced Thioredoxin-interacting Protein Ubiquitination and Proteasomal Degradation in Lung Fibroblasts: Implication in Pulmonary Fibrosis.

Taleb SJ, Ye Q, Baoyinna B, Dedad M, Pisini D, Parinandi NL, Cantley LC, Zhao J, Zhao Y
Journal of respiratory biology and translational medicine 2024 Mar;1(1)

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USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation.

Ma C, Wang D, Tian Z, Gao W, Zang Y, Qian L, Xu X, Jia J, Liu Z
Oncogene 2023 Jul;42(29):2249-2262

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High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer.

Wang H, Chen S, Kang W, Ding B, Cui S, Zhou L, Zhang N, Luo H, Wang M, Zhang F, Zhao Z, Guo Z, Wang C, Li L, Wang Z, Chen X, Wang Y
Discover oncology 2023 Feb 23;14(1):25

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MicroRNA profiling reveals miR-145-5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13.

Zhang Z, Deng K, Kang Z, Wang F, Fan Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Jul;36(7):e22370

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Deubiquitinase USP13 promotes the epithelial-mesenchymal transition and metastasis in gastric cancer by maintaining Snail protein.

Zhang T, Zheng J, Qiao L, Zhao W
Pathology, research and practice 2022 Jan;229:153705

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Establishment of a human induced pluripotent stem cell neuronal model for identification of modulators of A53T α-synuclein levels and aggregation.

Vajhøj C, Schmid B, Alik A, Melki R, Fog K, Holst B, Stummann TC
PloS one 2021;16(12):e0261536

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USP13 Deficiency Aggravates Cigarette-smoke-induced Alveolar Space Enlargement.

Gregory AD, Tran KC, Tamaskar AS, Wei J, Zhao J, Zhao Y
Cell biochemistry and biophysics 2021 Sep;79(3):485-491

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Lipopolysaccharide reduces USP13 stability through c-Jun N-terminal kinase activation in Kupffer cells.

Yu F, Li Y, Ye Q, Miao J, Taleb SJ, Zhao Y, Zhao J
Journal of cellular physiology 2021 Jun;236(6):4360-4368

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IL-37-induced activation of glycogen synthase kinase 3β promotes IL-1R8/Sigirr phosphorylation, internalization, and degradation in lung epithelial cells.

Li L, Wei J, Suber TL, Ye Q, Miao J, Li S, Taleb SJ, Tran KC, Tamaskar AS, Zhao J, Zhao Y
Journal of cellular physiology 2021 Aug;236(8):5676-5685

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Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.

Liao X, Li Y, Liu J, Zhang Y, Tan J, Kass DJ, Rojas M, Mallampalli RK, Zhao J, Zhao Y
Translational research : the journal of laboratory and clinical medicine 2020 Sep;223:15-24

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Potent USP10/13 antagonist spautin-1 suppresses melanoma growth via ROS-mediated DNA damage and exhibits synergy with cisplatin.

Guo J, Zhang J, Liang L, Liu N, Qi M, Zhao S, Su J, Liu J, Peng C, Chen X, Liu H
Journal of cellular and molecular medicine 2020 Apr;24(7):4324-4340

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USP13 serves as a tumor suppressor via the PTEN/AKT pathway in oral squamous cell carcinoma.

Qu Z, Zhang R, Su M, Liu W
Cancer management and research 2019;11:9175-9183

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Amplification of USP13 drives non-small cell lung cancer progression mediated by AKT/MAPK signaling.

Wu Y, Zhang Y, Liu C, Zhang Y, Wang D, Wang S, Wu Y, Liu F, Li Q, Liu X, Zaky MY, Yan D, Liu S
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Jun;114:108831

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The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation.

Li L, Wei J, Li S, Jacko AM, Weathington NM, Mallampalli RK, Zhao J, Zhao Y
EBioMedicine 2019 Jul;45:553-562

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Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.

Xia HG, Najafov A, Geng J, Galan-Acosta L, Han X, Guo Y, Shan B, Zhang Y, Norberg E, Zhang T, Pan L, Liu J, Coloff JL, Ofengeim D, Zhu H, Wu K, Cai Y, Yates JR, Zhu Z, Yuan J, Vakifahmetoglu-Norberg H
The Journal of cell biology 2015 Aug 31;210(5):705-16

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Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells.

Yoshioka Y, Ye YQ, Okada K, Taniguchi K, Yoshida A, Sugaya K, Onose J, Koshino H, Takahashi S, Yajima A, Yajima S, Abe N
PloS one 2013;8(12):e80931

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Chaperone-mediated autophagy degrades mutant p53.

Vakifahmetoglu-Norberg H, Kim M, Xia HG, Iwanicki MP, Ofengeim D, Coloff JL, Pan L, Ince TA, Kroemer G, Brugge JS, Yuan J
Genes & development 2013 Aug 1;27(15):1718-30

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP(USP13 antibody) at dilution of 1:400

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-USP13 (IP:16840-1-AP, 5ug; Detection:16840-1-AP 1:500) with HeLa cells lysate 2000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 16840-1-AP(USP13 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The USP13 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human USP13. This antibody recognizes human,mouse,rat antigen. The USP13 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

16840-1-AP.pdf

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