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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-46203 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46203, RRID:AB_10009435
- Product name
- Rabbit Polyclonal USP13 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP13 Antibody [NBP1-46203] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-USP13 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP13 was also immunoprecipitated by rabbit anti-USP13 antibody NBP1-46204, which recognizes a downstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP13 Antibody [NBP1-46203] - Detection of Human and Mouse USP13 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP13 antibody NBP1-46203 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP13 Antibody [NBP1-46203] - Detection of human and mouse USP13 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP13 antibody NBP1-46203 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: USP13 Antibody [NBP1-46203] - Detection of human USP13 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP13 antibody NBP1-46203 used for IP at 3 ug per reaction. USP13 was also immunoprecipitated by another rabbit anti-USP13 antibody. For blotting immunoprecipitated USP13, NBP1-46203 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP13 Antibody [NBP1-46203] - Human ovarian carcinoma (left) and mouse teratoma. Antibody used at a dilution of 1:5,000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP13 Antibody [NBP1-46203] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP13 (NBP1-46203). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP13 Antibody [NBP1-46203] - Detection of mouse USP13 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-USP13 (NBP1-46203). Detection: DAB
