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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-58802 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58802, RRID:AB_2272591
- Product name
- Rabbit Polyclonal UBE4B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE4B Antibody [NB100-58802] - Detection of Human and Mouse UBE4B on HeLa whole cell lysate using NB100-58802. UBE4B was also immunoprecipitated by rabbit anti-UBE4B antibody NB100-58803.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE4B Antibody [NB100-58802] - Detection of Human UBE4B by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE4B Antibody [NB100-58802] - Detection of mouse UBE4B by western blot. Samples: Nuclear extract (50 ug) from NIH 3T3, TCMK-1, and Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: UBE4B Antibody [NB100-58802] - Detection of human UBE4B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for IP at 6 ug per reaction. UBE4B was also immunoprecipitated by rabbit anti-UBE4B antibody NB100-58803. For blotting immunoprecipitated UBE4B, NB100-58802 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
