PA5-88581

antibody from Invitrogen Antibodies

Targeting: SHMT1 CSHMT, MGC15229, MGC24556, SHMT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88581

Provider

Invitrogen Antibodies

Product name

SHMT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FKVYQHQVVA NCRALSEALT ELGYKIVTGG SDNHLILVDL RSKGTDGGRA EKVLEACSIA CNKNTCPGDR SALRPSGLRL GTPALTSRGL LEKDFQKVAH FIHRGIELTL QIQSDTGVRA TLKEFKERLA GDKYQAAVQA LREEVESFAS LFPLPGLPDF; Positive Samples: 293T, HepG2, HT-29, K-562, A375, Mouse liver; Cellular Location: Cytoplasm

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

4.44 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SHMT1 in extracts of various cell lines using SHMT1 Polyclonal Antibody (Product # PA5-88581) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SHMT1 in extracts of various cell lines using SHMT1 Polyclonal Antibody (Product # PA5-88581) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SHMT1 was performed in U-2 OS cells using SHMT1 Polyclonal Antibody (Product # PA5-88581).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SHMT1 in paraffin-embedded rat kidney using SHMT1 Polyclonal Antibody (Product # PA5-88581) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SHMT1 in paraffin-embedded mouse kidney using SHMT1 Polyclonal Antibody (Product # PA5-88581) at a dilution of 1:100.