Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00003796-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003796-M01, RRID:AB_425517
- Product name
- KIF2 monoclonal antibody (M01), clone 2D4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KIF2.
- Antigen sequence
MVTSLNEDNESVTVEWIENGDTKGKEIDLESIFSL
NPDLVPDEEIEPSPETPPPPASSAKVNKIVKNRRT
VASIKNDPPSRDNRVVGSARARPSQFPEQSSSAQQ
NGSVSDISPVQAAKKEFGPPSRRKSNCVKEVEKLQ
EKREKRRLQQQELREKRAQDVDATNPNYEIMCMIR
DFRGSLDYRPLTTADPIDEHRICVCVRKRPLNKKE
TQMKDLDVITIPSKDVVMVHEPKQKVDLTRYLENQ
TFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMA
TCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALA
ARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDL
LNRKTKLRVLEDGKQQVQVVGLQEREVKCVEDVLK
LIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRKG
KLHGKFSLIDLAGNERGADTSSADRQTRLEGAEIN
KSLLALKECIRALGRNKPHTPFRASKLTQVLRDSF
IGENSRTCMIATISPGMASCENTLNTLRYANRVKE
LTVDPTAAGDVRPIMHHPPNQIDDLETQWGVGSSP
QRDDLKLLCEQNEEEVSPQLFTFHEAVSQMVEMEE
QVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVD
SYATQLEAILEQKIDILTELRDKVKSFRAALQEEE
QASKQINPKRPRAL- Isotype
- IgG
- Antibody clone number
- 2D4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Lysosomal positioning coordinates cellular nutrient responses.
Korolchuk VI, Saiki S, Lichtenberg M, Siddiqi FH, Roberts EA, Imarisio S, Jahreiss L, Sarkar S, Futter M, Menzies FM, O'Kane CJ, Deretic V, Rubinsztein DC
Nature cell biology 2011 Apr;13(4):453-60
Nature cell biology 2011 Apr;13(4):453-60
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KIF2 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to KIF2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol