16215-1-AP

antibody from Proteintech Group

Targeting: ACAT1 ACAT, THIL

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16215-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16215-1-AP, RRID:AB_2220210

Product name

ACAT1 antibody

Antibody type

Polyclonal

Description

KD/KO validated ACAT1 antibody (Cat. #16215-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Autophagy inhibition and reactive oxygen species elimination by acetyl-CoA acetyltransferase 1 through fused in sarcoma protein to promote prostate cancer.

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Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway.

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S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.

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Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.

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ACAT1 and Metabolism-Related Pathways Are Essential for the Progression of Clear Cell Renal Cell Carcinoma (ccRCC), as Determined by Co-expression Network Analysis.

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Human molecular genetics 2014 Mar 15;23(6):1399-412

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Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP(ACAT1 antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using ACAT1 antibody 16215-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 16215-1-AP(ACAT1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 16215-1-AP(ACAT1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue