Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
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Validation data
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- Product number
- STJ73206 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-ACAT1, Biotinylated antibody (253-266) {Biotin} (STJ73206)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-ACAT1, Biotinylated (253-266) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
DEEYKRVDFSKVPK.
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Biotinylated STJ72089 (0. 1µg/ml) staining of Mouse (A) and Rat (B) Liver lysates (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA