Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-82154 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACAT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VSATRTPIGS FLGSLSLLPA TKLGSIAIQG AIEKAGIPKE EVKEAYMGNV LQGGEGQAPT RQAVLGAGLP ISTPCTTINK VCASGMKAIM MASQSLMCGH QDVMVAGGME SMSNVPYVMN RGSTPYGGVK LEDLIVKDGL TD
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection.
Hashimoto Y, Sheng X, Murray-Nerger LA, Cristea IM
Nature communications 2020 Feb 10;11(1):806
Nature communications 2020 Feb 10;11(1):806
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACAT1 by a ACAT1 polyclonal antibody (Product # PA5-82154). Analysis in human liver tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACAT1 by a ACAT1 polyclonal antibody (Product # PA5-82154). Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ACAT1 in A-431 cells using a ACAT1 polyclonal antibody (Product # PA5-82154). The analysis shows localization to mitochondria.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ACAT1 in human kidney using a ACAT1 polyclonal antibody (Product # PA5-82154). The analysis shows strong positivity in mitochondria in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ACAT1 in human liver using a ACAT1 polyclonal antibody (Product # PA5-82154). The analysis shows strong positivity in mitochondria in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ACAT1 in human cerebellum using a ACAT1 polyclonal antibody (Product # PA5-82154). The analysis shows strong positivity in mitochondria in purkinje cells.