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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- Ab087103 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- ACAT1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human ACAT1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.ApplicationWB: 0.5-2µg/mL;IHC: 5-20µg/mL;ICC/IF: 5-20µg/mL;Protein FunctionPlays a major role in ketone body metabolism.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 111-427- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ACAT1 Antibody (Ab087103) - Western Blot All lanes: ACAT1 Antibody (Ab087103) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 40 kDa Exposure time: 19.8 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ACAT1 Antibody (Ab087103) - IF FITC staining on IF Samples: Human Hep G2 cells Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103) Second Ab: 1.5μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ACAT1 Antibody (Ab087103) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ACAT1 Antibody (Ab087103) - IHC DAB staining on IHC Samples: Human Lymph node Tissue Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
