Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-89285 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89285, RRID:AB_11005222
- Product name
- Rabbit Polyclonal ACAT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ACAT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Altering lipid droplet homeostasis affects Coxiella burnetii intracellular growth.
Retinal Hypercholesterolemia Triggers Cholesterol Accumulation and Esterification in Photoreceptor Cells.
Ethanol exposure induces the cancer-associated fibroblast phenotype and lethal tumor metabolism: implications for breast cancer prevention.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Mulye M, Zapata B, Gilk SD
PloS one 2018;13(2):e0192215
PloS one 2018;13(2):e0192215
Retinal Hypercholesterolemia Triggers Cholesterol Accumulation and Esterification in Photoreceptor Cells.
Saadane A, Mast N, Dao T, Ahmad B, Pikuleva IA
The Journal of biological chemistry 2016 Sep 23;291(39):20427-39
The Journal of biological chemistry 2016 Sep 23;291(39):20427-39
Ethanol exposure induces the cancer-associated fibroblast phenotype and lethal tumor metabolism: implications for breast cancer prevention.
Sanchez-Alvarez R, Martinez-Outschoorn UE, Lin Z, Lamb R, Hulit J, Howell A, Sotgia F, Rubin E, Lisanti MP
Cell cycle (Georgetown, Tex.) 2013 Jan 15;12(2):289-301
Cell cycle (Georgetown, Tex.) 2013 Jan 15;12(2):289-301
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in human liver tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar pattern to independent antibody NBP1-89284 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar protein distribution across tissues to independent antibody NBP1-89284 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum shows strong positivity in mitochondria in purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.