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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
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- Product number
- HPA004428 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA004428, RRID:AB_1078088
- Product name
- Anti-ACAT1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ACAT1, Gene description: acetyl-CoA acetyltransferase 1, Alternative Gene Names: ACAT, THIL, Validated applications: ICC, IHC, WB, Uniprot ID: P24752, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Prostate cancer castrate resistant progression usage of non-canonical androgen receptor signaling and ketone body fuel
Epigenetic Inactivation of Acetyl-CoA Acetyltransferase 1 Promotes the Proliferation and Metastasis in Nasopharyngeal Carcinoma by Blocking Ketogenesis
Dysregulation of Ketone Body Metabolism Is Associated With Poor Prognosis for Clear Cell Renal Cell Carcinoma Patients
Muscle PGC-1α is required for long-term systemic and local adaptations to a ketogenic diet in mice
Skeletal muscle PGC‐1α modulates systemic ketone body homeostasis and ameliorates diabetic hyperketonemia in mice
Retinal Hypercholesterolemia Triggers Cholesterol Accumulation and Esterification in Photoreceptor Cells
Ethanol exposure induces the cancer-associated fibroblast phenotype and lethal tumor metabolism
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Labanca E, Bizzotto J, Sanchis P, Anselmino N, Yang J, Shepherd P, Paez A, Antico-Arciuch V, Lage-Vickers S, Hoang A, Tang X, Raso M, Titus M, Efstathiou E, Cotignola J, Araujo J, Logothetis C, Vazquez E, Navone N, Gueron G
Oncogene 2021;40(44):6284-6298
Oncogene 2021;40(44):6284-6298
Epigenetic Inactivation of Acetyl-CoA Acetyltransferase 1 Promotes the Proliferation and Metastasis in Nasopharyngeal Carcinoma by Blocking Ketogenesis
Lu Y, Zhou X, Zhao W, Liao Z, Li B, Han P, Yang Y, Zhong X, Mo Y, Li P, Huang G, Xiao X, Zhang Z, Zhou X
Frontiers in Oncology 2021;11
Frontiers in Oncology 2021;11
Dysregulation of Ketone Body Metabolism Is Associated With Poor Prognosis for Clear Cell Renal Cell Carcinoma Patients
Cui W, Luo W, Zhou X, Lu Y, Xu W, Zhong S, Feng G, Liang Y, Liang L, Mo Y, Xiao X, Huang G, Matskova L, Zhang Z, Li P, Zhou X
Frontiers in Oncology 2019;9
Frontiers in Oncology 2019;9
Muscle PGC-1α is required for long-term systemic and local adaptations to a ketogenic diet in mice
Schnyder S, Svensson K, Cardel B, Handschin C
American Journal of Physiology-Endocrinology and Metabolism 2017;312(5):E437-E446
American Journal of Physiology-Endocrinology and Metabolism 2017;312(5):E437-E446
Skeletal muscle PGC‐1α modulates systemic ketone body homeostasis and ameliorates diabetic hyperketonemia in mice
Svensson K, Albert V, Cardel B, Salatino S, Handschin C
The FASEB Journal 2016;30(5):1976-1986
The FASEB Journal 2016;30(5):1976-1986
Retinal Hypercholesterolemia Triggers Cholesterol Accumulation and Esterification in Photoreceptor Cells
Saadane A, Mast N, Dao T, Ahmad B, Pikuleva I
Journal of Biological Chemistry 2016;291(39):20427-20439
Journal of Biological Chemistry 2016;291(39):20427-20439
Ethanol exposure induces the cancer-associated fibroblast phenotype and lethal tumor metabolism
Sanchez-Alvarez R, Martinez-Outschoorn U, Lin Z, Lamb R, Hulit J, Howell A, Sotgia F, Rubin E, Lisanti M
Cell Cycle 2014;12(2):289-301
Cell Cycle 2014;12(2):289-301
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251
Journal of Proteomics 2012;75(7):2236-2251
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
