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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
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- Product number
- HPA007569 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA007569, RRID:AB_1844482
- Product name
- Anti-ACAT1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ACAT1, Gene description: acetyl-CoA acetyltransferase 1, Alternative Gene Names: ACAT, THIL, Validated applications: ICC, IHC, WB, Uniprot ID: P24752, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references The expression of ACAT1 in oral squamous cell carcinoma and the adjacent pre-tumour tissue
Ketone body utilization drives tumor growth and metastasis
Ketone bodies and two-compartment tumor metabolism: Stromal ketone production fuels mitochondrial biogenesis in epithelial cancer cells
Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection
Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy
Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Firouzpour H, Shokrolahi S, Bourova-Flin E, Derakhshan S, Shahsavari Z, Karimi A, Mir Mohammad Sadeghi H, Goudarzi A
BioMedicine 2022;12(4):55-62
BioMedicine 2022;12(4):55-62
Ketone body utilization drives tumor growth and metastasis
Martinez-Outschoorn U, Lin Z, Whitaker-Menezes D, Howell A, Sotgia F, Lisanti M
Cell Cycle 2014;11(21):3964-3971
Cell Cycle 2014;11(21):3964-3971
Ketone bodies and two-compartment tumor metabolism: Stromal ketone production fuels mitochondrial biogenesis in epithelial cancer cells
Martinez-Outschoorn U, Lin Z, Whitaker-Menezes D, Howell A, Lisanti M, Sotgia F
Cell Cycle 2014;11(21):3956-3963
Cell Cycle 2014;11(21):3956-3963
Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection
Andersson S, Konrad A, Ashok N, Pontén F, Hober S, Asplund A
Journal of Histochemistry & Cytochemistry 2013;61(11):773-784
Journal of Histochemistry & Cytochemistry 2013;61(11):773-784
Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy
Chang H, Olson L, Schwartz K
Nutrition & Metabolism 2013;10(1):47
Nutrition & Metabolism 2013;10(1):47
Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection
Andersson S, Konrad A, Ashok N, Ponten F, Hober S, Asplund A
Journal of Histochemistry & Cytochemistry 2013 October;61(11):773-784
Journal of Histochemistry & Cytochemistry 2013 October;61(11):773-784
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251
Journal of Proteomics 2012;75(7):2236-2251
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
