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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-26135 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GADD45G Monoclonal Antibody (OTI2F12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2F12
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed on GBM8401 cells using 500 µg of protein. Antigen-antibody complexes were formed by incubating the sample with GADD45G monoclonal antibody (Product # MA5-26135) antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed on GBM8401 cells using 500 µg of protein. Antigen-antibody complexes were formed by incubating the sample with GADD45G monoclonal antibody (Product # MA5-26135) antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed on CRC cells using 500 µg of protein. Antigen-antibody complexes were formed by incubating the sample with GADD45G monoclonal antibody (Product # MA5-26135) antibody. The immunoprecipitates were collected using protein A/G agarose beads, boiled and washed to pellet the beads.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GADD45G in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with GADD45G (Product # MA5-26135) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GADD45G in human NPC cells. Samples were separated by SDS-PAGE and probed with GADD45G (Product # MA5-26135) monoclonal antibody and HRP conjugated secondary antibody at a dilution of 1:20,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GADD45G in COS7 cells. Cells were transfected with a plasmid overexpressing GADD45G and probed with a GADD45G monoclonal antibody (Product # MA5-26135).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a GADD45G monoclonal antibody (Product # MA5-26135).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a GADD45G monoclonal antibody (Product # MA5-26135).
