AP2417B

antibody from Abgent

Targeting: FDFT1 SQS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2417B

Provider

Abgent

Proper citation

Abgent Cat#AP2417b, RRID:AB_353150

Product name

FDFT1 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Proteomic analysis of doxorubicin-induced changes in the proteome of HepG2cells combining 2-D DIGE and LC-MS/MS approaches.

Hammer E, Bien S, Salazar MG, Steil L, Scharf C, Hildebrandt P, Schroeder HW, Kroemer HK, Völker U, Ritter CA
Proteomics 2010 Jan;10(1):99-114

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Docosahexaenoic acid activates some SREBP-2 targets independent of cholesterol and ER stress in SW620 colon cancer cells.

Størvold GL, Fleten KG, Olsen CG, Follestad T, Krokan HE, Schønberg SA
Lipids 2009 Aug;44(8):673-83

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-FDFT1 Antibody (Center) (Cat.#AP2417b) pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2417b) in A375 cell line lysate. FDFT1(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of FDFT1 (arrow) using rabbit polyclonal FDFT1 Antibody (A155) (Cat.#AP2417b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FDFT1 gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with FDFT1 Antibody (Center) (Cat.#AP2417b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100