Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP2417B - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2417b, RRID:AB_353150
- Product name
- FDFT1 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Proteomic analysis of doxorubicin-induced changes in the proteome of HepG2cells combining 2-D DIGE and LC-MS/MS approaches.
Docosahexaenoic acid activates some SREBP-2 targets independent of cholesterol and ER stress in SW620 colon cancer cells.
Hammer E, Bien S, Salazar MG, Steil L, Scharf C, Hildebrandt P, Schroeder HW, Kroemer HK, Völker U, Ritter CA
Proteomics 2010 Jan;10(1):99-114
Proteomics 2010 Jan;10(1):99-114
Docosahexaenoic acid activates some SREBP-2 targets independent of cholesterol and ER stress in SW620 colon cancer cells.
Størvold GL, Fleten KG, Olsen CG, Follestad T, Krokan HE, Schønberg SA
Lipids 2009 Aug;44(8):673-83
Lipids 2009 Aug;44(8):673-83
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-FDFT1 Antibody (Center) (Cat.#AP2417b) pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2417b) in A375 cell line lysate. FDFT1(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of FDFT1 (arrow) using rabbit polyclonal FDFT1 Antibody (A155) (Cat.#AP2417b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FDFT1 gene.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with FDFT1 Antibody (Center) (Cat.#AP2417b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100