PA5-28479

antibody from Invitrogen Antibodies

Targeting: STAU1 PPP1R150, STAU

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28479

Provider

Invitrogen Antibodies

Product name

Staufen Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HepG2, Molt-4, Raji, Neuro2A, 293 (input), Stau1-IP (GTX106566), Post-IP lysate from control rabbit IgG-IP, Post-IP lysate from Stau1-IP.

Concentration

0.93 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Staufen using A) 293 (input) (B) control rabbit IgG-IP (C) Stau1-IP (D) Post-IP lysate from control rabbit IgG-IP and E) Post-IP lysate from Stau1-IP. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Staufen polyclonal antibody (Product # PA5-28479) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Staufen using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Staufen polyclonal antibody (Product # PA5-28479) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Staufen Polyclonal Antibody (Product # PA5-28479). Sample (30 µg of whole cell lysate). Lane A: Neuro2A. Staufen Polyclonal Antibody (Product # PA5-28479) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Staufen Polyclonal Antibody (Product # PA5-28479). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen Polyclonal Antibody (Product # PA5-28479) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Staufen was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Staufen Polyclonal Antibody (Product # PA5-28479) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.