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- Immunohistochemistry [1]
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- Product number
- PA5-53820 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NMVVADVYNH RFHKIFQMDE GLNHIMPRDD IFVYEVCSTS VDGSECVTLP VYFRERKSRP SSTSSASALY GQPLLLSVPK HKLTLESLYQ AVCDRISRYV KQPLPDEFGS SPL
- Concentration
- 0.3 mg/mL
Submitted references Ubiquitously specific protease 4 inhibitor-Vialinin A attenuates inflammation and fibrosis in S100-induced hepatitis mice through Rheb/mTOR signalling.
Xu J, Chen D, Jin L, Chen Z, Tu Y, Huang X, Xue F, Xu J, Chen M, Wang X, Chen Y
Journal of cellular and molecular medicine 2021 Jan;25(2):1140-1150
Journal of cellular and molecular medicine 2021 Jan;25(2):1140-1150
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP4 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an USP4 Polyclonal Antibody (Product # PA5-53820).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP4 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an USP4 Polyclonal Antibody (Product # PA5-53820).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of USP4 in human cell line A-431 shows positivity in plasma membrane & cytoplasm. Samples were probed using an USP4 Polyclonal Antibody (Product # PA5-53820).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of USP4 in human rectum using an USP4 Polyclonal Antibody (Product # PA5-53820) shows moderate cytoplasmic positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 The expression of USP4 and the mRNA levels of inflammatory cytokines in the liver of AIH mice and AML12 cells. Panel A shows representative immunohistochemistry staining (magnification, x400) of USP4 and the USP4 expression score in liver of normal mice and AIH model. Panel B displays the histogram of relative mRNA levels of USP4, IL-1 and IL-6 in the liver from each group. Panel C shows the histogram of relative mRNA level from AML12 cells treated with LPS plus or minus Vialinin A or specific inhibitory siRNA of UPS4. GAPDH used as loading control. The data are presented as mean +- SDM from 6 mice or 3 independent experiments of cells. ** P < .01 and *** P < .001
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 The protein levels of USP4 and fibrotic cytokines in mice and AML12 cells. Panel A shows the representative Western blot and histogram of proteins for USP4, COL-1, alpha-SMA, TGF-beta and FN14 in from each group. Panel B displays the typical Western blot and histogram of proteins of fibrotic cytokines from AML12 cells treated with LPS plus or minus Vialinin A. Panel C shows the typical Western blot and histogram of proteins different fibrotic cytokines proteins from AML12 cells treated with LPS specific inhibitory siRNA of UPS4. GAPDH used as loading control. The data represents mean +- SDM from 6 mice or 3 independent experiments from cells. * P < .05, ** P < .01 and *** P < .001
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 Immunoprecipitation experiments shows USP4 and Vialinini A involved in regulation of Rheb deubiquitination. Panel A shows the Rheb protein level in AML12 cells treated with 5 umol/L LPS for 24 h and further treated with different protein inhibitors individually or in combination for 6 h of CHX (100 mg/mL), lysosomal inhibitor chloroquine (50 umol/L) with and proteasome inhibitor MG132 (5 umol/L). Panel B shows the immunoprecipated with USP4 and immunoblot with USP4 and Rheb with immunoprecipitaed proteins. Panel C displays the immunoprecipation experiments with proteasome inhibitor MG132 and LPS as well as with and without specific inhibitory siRNA of USP4. Protein was immunoprecipitated with antibody of Rheb and immunoblotted with Ubiquitin antibody. Panel d shows the same immunoprecipitation experiments as in panel C except with Vialinin A instead of specific inhibitory siRNA of USP4. The right Panel displays the histogram of (C) and (D). Panel e shows USP4 mRNA level from healthy and AIH patients. The data are presented as mean +- SDM from 6 mice of 3 independent experiments of cells. ** P < .01 and *** P < .001
