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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- NB100-2868 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2868, RRID:AB_2213521
- Product name
- Rabbit Polyclonal USP4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A cytosolic ATM/NEMO/RIP1 complex recruits TAK1 to mediate the NF-kappaB and p38 mitogen-activated protein kinase (MAPK)/MAPK-activated protein 2 responses to DNA damage.
Yang Y, Xia F, Hermance N, Mabb A, Simonson S, Morrissey S, Gandhi P, Munson M, Miyamoto S, Kelliher MA
Molecular and cellular biology 2011 Jul;31(14):2774-86
Molecular and cellular biology 2011 Jul;31(14):2774-86
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: USP4 Antibody [NB100-2868] - Detection of Human USP4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2868 used for IP at 3 mcg/mg lysate. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2869, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.
