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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-2869 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2869, RRID:AB_2213522
- Product name
- Rabbit Polyclonal USP4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome.
Song EJ, Werner SL, Neubauer J, Stegmeier F, Aspden J, Rio D, Harper JW, Elledge SJ, Kirschner MW, Rape M
Genes & development 2010 Jul 1;24(13):1434-47
Genes & development 2010 Jul 1;24(13):1434-47
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP4 Antibody [NB100-2869] - Detection of Human USP4 on HeLa whole cell lysate using NB100-2869. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2868.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP4 Antibody [NB100-2869] - Detection of Human and Mouse USP4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: USP4 Antibody [NB100-2869] - Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for IP at 6 ug per reaction. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2868. For blotting immunoprecipitated USP4, NB100-2869 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
