HPA018152

antibody from Atlas Antibodies

Targeting: ARFGAP2 FLJ14576, IRZ, Zfp289, ZNF289

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA018152

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA018152, RRID:AB_1844979

Product name

Anti-ARFGAP2

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LGMGLVSRSSVSHSVLSEMQVIEQETPVSAKSSRS
QLDLFDDVGTFASGPPKYKDNPFSLGESFGSRWDT
DAAWGMDRVEEKEPEVTISSIRPISERATNRREVE
SRSSGLESSEARQKFAGAKAISSD


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line MCF-7 and human cell line SK-MEL-30.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, kidney, pancreas and testis using Anti-ARFGAP2 antibody HPA018152 (A) shows similar protein distribution across tissues to independent antibody HPA016649 (B).

Antibody #2 product nr

HPA016649

Antibody provider

Atlas Antibodies

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-ARFGAP2 antibody HPA018152.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas using Anti-ARFGAP2 antibody HPA018152.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-ARFGAP2 antibody HPA018152.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-ARFGAP2 antibody HPA018152.

Sample type

HUMAN