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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- RQ5793 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Afm Antibody / Afamin
- Antibody type
- Polyclonal
- Description
- This highly specific Afm antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with mouse and rat samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Afm antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat 1) heart, 2) liver, 3) kidney, 4) spleen and mouse 5) spleen, 6) thymus and 7) ANA-1 lysate with Afm antibody. Predicted molecular weight: ~69/87 kDa (unmodified/glycosylated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat liver with Afm antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse spleen cells with Afm antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afm antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse HEPA1-6 cells with Afm antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afm antibody.
