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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00010590-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010590-M01, RRID:AB_425857
- Product name
- SCGN monoclonal antibody (M01), clone 2G7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SCGN.
- Antigen sequence
MDSSREPTLGRLDAAGFWQVWQRFDADEKGYIEEK
ELDAFFLHMLMKLGTDDTVMKANLHKVKQQFMTTQ
DASKDGRIRMKELAGMFLSEDENFLLLFRRENPLD
SSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLH
HKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLA
RILALQENFLLQFKMDACSTEERKRDFEKIFAYYD
VSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKF
REILLRHCDVNKDGKIQKSELALCLGLKINP- Isotype
- IgG
- Antibody clone number
- 2G7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma beta-cells (INS-1).
Proteomic analysis of normal human nasal mucosa: establishment of a two-dimensional electrophoresis reference map.
Bazwinsky-Wutschke I, Wolgast S, Mühlbauer E, Peschke E
Histochemistry and cell biology 2010 Aug;134(2):115-27
Histochemistry and cell biology 2010 Aug;134(2):115-27
Proteomic analysis of normal human nasal mucosa: establishment of a two-dimensional electrophoresis reference map.
Lee JY, Byun JY, Lee SH
Clinical biochemistry 2009 May;42(7-8):692-700
Clinical biochemistry 2009 May;42(7-8):692-700
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SCGN is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SCGN on formalin-fixed paraffin-embedded human yolk sac tumor. [antibody concentration 6 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
