Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-21847 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRPSAP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse liver. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (93%), Chicken (97%), Chimpanzee (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRPSAP2 Polyclonal Antibody (Product # PA5-21847). Sample (30 µg whole cell lysate). A: HeLa S3. 7.5% SDS PAGE. PRPSAP2 Polyclonal Antibody (Product # PA5-21847) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRPSAP2 Polyclonal Antibody (Product # PA5-21847). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 12% SDS PAGE. PRPSAP2 Polyclonal Antibody (Product # PA5-21847) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRPSAP2 in methanol-fixed HeLa cells using a PRPSAP2 polyclonal antibody (Product # PA5-21847) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using PRPSAP2 antibody (Product # PA5-21847) at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PRPSAP2 was performed in paraffin-embedded mouse duodenum tissue using PRPSAP2 Polyclonal Antibody (Product # PA5-21847) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PRPSAP2 (Product # PA5-21847) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.