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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-27798 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF3h Monoclonal Antibody (GT35512)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A431 Predicted Reactivity: Rat (99%), Xenopus laevis (86%), Chicken (95%), Bovine (98%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT35512
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3H in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with eIF3H monoclonal antibody (Product # MA5-27798) using a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF3H/EIF3S3 antibody [GT35512] detects eIF3H/EIF3S3 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF3H/EIF3S3 protein stained by eIF3H/EIF3S3 antibody [GT35512] (Product # MA5-27798) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF3H/EIF3S3 antibody [GT35512] detects eIF3H/EIF3S3 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EIF3H/EIF3S3 antibody [GT35512] (Product # MA5-27798) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
