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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- PA5-89202 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF3h Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NCFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ GSLSLKAYRL TPKLMEVCKE KDFSPEALKK ANITFEYMFE EVPIVIKNSH LINVLMWELE KKSAVADKHE LLSLASSNHL GKNLQLLMDR VDEMSQDIVK YNTYMRNTSK QQQQKHQYQQ RRQQENMQRQ SRGEPPLPEE DLSKLFKPPQ PPARMDSLLI AGQINTYCQN IKEFTAQNLG K; Positive Samples: HL-60, HT-29, NIH/3T3, HeLa, Jurkat, Mouse spleen, Mouse testis, Mouse heart, Rat spleen; Cellular Location: Cytoplasm
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.02 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3h in extracts of various cell lines. Samples were incubated with eIF3h Polyclonal antibody (Product # PA5-89202) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of eIF3h was performed in MCF7 cells using eIF3h Polyclonal Antibody (Product # PA5-89202).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of eIF3h in 200 μg extracts of Jurkat cells. Samples were precipitated with 1 μg eIF3h Polyclonal antibody (Product # PA5-89202). Western blot was performed from the immunoprecipitate using eIF3h Polyclonal antibody (Product # PA5-89202) at a dilution of 1:1,000.
