PA5-82257

antibody from Invitrogen Antibodies

Targeting: EPB41L2 4.1-G, 4.1G

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-82257

Provider

Invitrogen Antibodies

Product name

EPB41L2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ESQSSLRRQK REKETSESRG ISRFIPPWLK KQKSYTLVVA KDGGDKKEPT QAVVEEQVLD KEEPLPEEQR QAKGDAEEMA QKKQEIKVEV KEEKPSVSKE EKPSVSKVEM QPTELVSKER EEKVKETQED KLEGGA

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity.

Troyanovsky RB, Indra I, Kato R, Mitchell BJ, Troyanovsky SM
The Journal of biological chemistry 2021 Nov;297(5):101289

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EPB41L2 in U-2 OS cells using a EPB41L2 polyclonal antibody (Product # PA5-82257). The analysis shows localization to plasma membrane & cell junctions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of EPB41L2 in human rectum using EPB41L2 Polyclonal Antibody (Product # PA5-82257) shows strong membranous and cytoplasmic positivity in glandular cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Schematic representation and major characteristics of the sLUR point mutants. A , the conserved segments of 17 LRRs of Scribble are organized in numbered columns. These segments, with a consensus sequence LxxLxLxxNxL, form a beta-sheet of the concave surface of Scribble LRR domain. Position of the first residue in each segment is indicated by a corresponding number. The consensus residues ( arrows ) in these segments form the core of the protein, whereas other residues are solvent-exposed. The residues are color-coded according to a presented chart. All residues that are identical in Scribble, Erbin, and Lano are marked by a single letter code. The residues tested by site-directed mutagenesis are marked by dots . The mutations that abolish dimerization are circled . B , BN-PAGE of the lysates obtained from DLD20-2 cells expressing control sLUR-517GFP (sLUR-517GFP) and its mutants (expressed by corresponding mutations). Data are representative of three independent experiments. Note that some of the mutations completely abolish formation of the high molecular weight dimeric form of sLUR-517GFP. C , representative confocal optical sections of LAP protein-deficient DLD20-2 cells (as a negative control), DLD20-2 cells expressing sLUR-517GFP (as positive control), and three of the dimerization incompetent mutants. Note that both mutants containing W203A mutations (W203A and triple mutation W203A/L249A/K272A) are localized at both apical and basolateral membranes. Bar, 10 m