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- Antibody Data
- Antigen structure
- References [1]
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- Other assay [1]
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- Product number
- R1517 - Provider product page
- Provider
- OriGene
- Product name
- DDB2 rabbit polyclonal antibody, Serum
- Antibody type
- Polyclonal
- Description
- DDB2 rabbit polyclonal antibody, Serum
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DDB2
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 85.0 mg/ml (by refractometry)
Submitted references Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC.
Luijsterburg MS, Goedhart J, Moser J, Kool H, Geverts B, Houtsmuller AB, Mullenders LH, Vermeulen W, van Driel R
Journal of cell science 2007 Aug 1;120(Pt 15):2706-16
Journal of cell science 2007 Aug 1;120(Pt 15):2706-16
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Supportive validation
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- OriGene (provider)
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- Experimental details
- Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S. Jentsch, see references above.
- Validation comment
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