PA5-63568

antibody from Invitrogen Antibodies

Targeting: DDB2 DDBB, FLJ34321, UV-DDB2, XPE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-63568

Provider

Invitrogen Antibodies

Product name

Anti-DDB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GPSRRCDSDC LWVGLAGPQI LPPCRSIVRT LHQHKLGRAS WPSVQQGLQQ SFLHTLDSYR ILQKAAPFDR RATSLAWHPT HPSTVAVGSK GGDIMLWNFG IKDKPTFIKG IGAGGSITGL KFNPLNTNQF YASSMEGTTR Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 76%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDB2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG. Samples were probed using a DDB2 Polyclonal Antibody (Product # PA5-63568).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDB2 in human skin shows strong nuclear positivity in epidermal cells. Samples were probed using a DDB2 Polyclonal Antibody (Product # PA5-63568).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDB2 in human esophagus and skeletal muscle tissues using DDB2 Polyclonal Antibody (Product # PA5-63568). Corresponding DDB2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDB2 in human skin using DDB2 Polyclonal Antibody (Product # PA5-63568) shows nuclear positivity in epidermal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDB2 in human esophagus using DDB2 Polyclonal Antibody (Product # PA5-63568) shows nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DDB2 in human skeletal muscle using DDB2 Polyclonal Antibody (Product # PA5-63568) shows no positivity in myocytes as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DDB2 in human cell line HEK 293 shows positivity in cell junctions & nucleus but excluded from the nucleoli. Samples were probed using a DDB2 Polyclonal Antibody (Product # PA5-63568).