Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-63568 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DDB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GPSRRCDSDC LWVGLAGPQI LPPCRSIVRT LHQHKLGRAS WPSVQQGLQQ SFLHTLDSYR ILQKAAPFDR RATSLAWHPT HPSTVAVGSK GGDIMLWNFG IKDKPTFIKG IGAGGSITGL KFNPLNTNQF YASSMEGTTR Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 76%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDB2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG. Samples were probed using a DDB2 Polyclonal Antibody (Product # PA5-63568).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DDB2 in human skin shows strong nuclear positivity in epidermal cells. Samples were probed using a DDB2 Polyclonal Antibody (Product # PA5-63568).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DDB2 in human esophagus and skeletal muscle tissues using DDB2 Polyclonal Antibody (Product # PA5-63568). Corresponding DDB2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DDB2 in human skin using DDB2 Polyclonal Antibody (Product # PA5-63568) shows nuclear positivity in epidermal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DDB2 in human esophagus using DDB2 Polyclonal Antibody (Product # PA5-63568) shows nuclear positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DDB2 in human skeletal muscle using DDB2 Polyclonal Antibody (Product # PA5-63568) shows no positivity in myocytes as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DDB2 in human cell line HEK 293 shows positivity in cell junctions & nucleus but excluded from the nucleoli. Samples were probed using a DDB2 Polyclonal Antibody (Product # PA5-63568).