Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-34577AF700 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal alpha-Fetoprotein/AFP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine, Simian
- Host
- Mouse
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: alpha-Fetoprotein/AFP Antibody (C3) [Alexa Fluor® 700] [NBP2-34577AF700] - An intracellular stain was performed on HepG2 cells with alpha-Fetoprotein/AFP Antibody [C3] NBP2-34577AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.