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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- TA809081 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA809081, RRID:AB_2629001
- Product name
- AFP mouse monoclonal antibody, clone OTI2F11 (formerly 2F11)
- Antibody type
- Monoclonal
- Description
- AFP mouse monoclonal antibody, clone OTI2F11 (formerly 2F11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AFP
- Isotype
- IgG
- Antibody clone number
- OTI2F11
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AFP mouse monoclonal antibody (TA809081) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP(RC206622).(1:100)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using anti-AFP mouse monoclonal antibody (TA809081).(1:100)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120?°C for 3 min, TA809081)(1:150)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AFP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809081)(1:150)
- Validation comment
- IHC
