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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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Validation data
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- Product number
- TA809082 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA809082, RRID:AB_2629002
- Product name
- AFP mouse monoclonal antibody, clone OTI4G9 (formerly 4G9)
- Antibody type
- Monoclonal
- Description
- AFP mouse monoclonal antibody, clone OTI4G9 (formerly 4G9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AFP
- Isotype
- IgG
- Antibody clone number
- OTI4G9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.(1:2000)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AFP mouse monoclonal antibody (TA809082) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP(RC206622).(1:100)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-AFP mouse monoclonal antibody (TA809082).(1:100)
- Validation comment
- IF
