TA809084

antibody from OriGene

Targeting: AFP FETA, HPAFP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA809084

Provider

OriGene

Proper citation

OriGene Cat#TA809084, RRID:AB_2629004

Product name

AFP mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)

Antibody type

Monoclonal

Description

AFP mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)

Host

Mouse

Conjugate

Unconjugated

Epitope

AFP

Isotype

IgG

Antibody clone number

OTI3F1

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.(1:2000)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:500)

Validation comment

WB