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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- UM500056 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500056, RRID:AB_2629069
- Product name
- AFP mouse monoclonal antibody, clone UMAB66
- Antibody type
- Monoclonal
- Description
- AFP mouse monoclonal antibody, clone UMAB66
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AFP
- Isotype
- IgG
- Antibody clone number
- UMAB66
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody (Clone UMAB66).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using AFP mouse monoclonal antibody (UM500056, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human embryonic liver tissue using anti-AFP mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min in pressure chamber/cooker. UM500056 diluted 1:100 and detection shown with HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in the hepatocytes.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human placenta using anti-AFP clone UMAB66 mouse monoclonal antibody at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. UM500056 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous, cytoplasmic and rare nuclear protein in the trophoblast cells. This protein is also seen as secreted protein.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-AFP mouse monoclonal antibody (UM500056). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
